At5g52620 Antibody

Overview of At5g52620 Antibody

The At5g52620 antibody is a polyclonal reagent developed to detect the protein encoded by the At5g52620 gene in Arabidopsis thaliana. This gene is annotated under UniProt accession Q9LTF5, though its precise biological function remains under investigation. The antibody is widely used in plant molecular biology to study protein expression, localization, and interactions .

3.1. Protein Expression Profiling

The antibody enables detection of the At5g52620 protein in plant tissues, aiding in studies of its expression under varying physiological or stress conditions. For example, similar antibodies have been used to map tissue-specific protein localization in roots, leaves, and floral organs .

3.2. Functional Studies

While the exact role of At5g52620 is unclear, homologs in Arabidopsis are often involved in metabolic pathways or stress responses. This antibody could facilitate loss-of-function or overexpression studies to elucidate its biological significance .

3.3. Comparative Analyses

Cross-reactivity studies with antibodies like anti-DEFA5 (clones 1A8/4F5) highlight the importance of rigorous validation to avoid off-target binding, a consideration critical for At5g52620 research .

Limitations and Future Directions

• Specificity Gaps: No peer-reviewed studies directly validating At5g52620 antibody performance were identified. Cross-reactivity with unrelated proteins (e.g., AMPD2 or TRIM28, as seen with anti-GR clone 5E4) remains a potential concern .

• Functional Data: The absence of published knockdown or phenotypic data for At5g52620 limits mechanistic insights.