At5g52880 Antibody

Definition and Overview of At5g52880 Antibody

The At5g52880 Antibody (product code: CSB-PA863806XA01DOA) is a polyclonal antibody directed against the F-box family protein encoded by the AT5G52880 gene in Arabidopsis thaliana (Mouse-ear cress). This antibody is designed for immunological detection and characterization of the target protein in plant research contexts.

Source: CUSABIO product catalog .

Research Applications and Functional Insights

The At5g52880 Antibody is primarily utilized in plant molecular biology to study F-box proteins, which are critical components of the SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complexes. These complexes regulate protein degradation via the ubiquitin-proteasome system, influencing developmental processes such as cell differentiation and stress responses .

Key Findings from Plant Studies

1. Immunoprecipitation (IP) and Protein Interactions:

   • The antibody has been employed in IP experiments to isolate the AT5G52880 protein from Arabidopsis cell lysates. This approach helps identify interacting partners, such as transcription factors or regulatory proteins involved in development (e.g., CUC1, a key regulator of leaf morphology) .

   • Example: In a study investigating the CUP-SHAPED COTYLEDON1 (CUC1) regulatory network, the At5g52880 Antibody was used to immunoprecipitate nuclear and cytoplasmic interactors, revealing its role in modulating protein complexes during development .

2. Role in Ubiquitination Pathways:

   • F-box proteins like AT5G52880 serve as substrate receptors for SCF complexes, targeting specific proteins for degradation. The antibody enables researchers to track the localization and activity of these proteins in vivo.

3. Cross-Species Utility:
   While optimized for Arabidopsis, the antibody’s specificity for conserved F-box domains suggests potential utility in studying orthologous proteins in other plant species.

Technical Considerations and Limitations

• Specificity: The antibody is validated for Arabidopsis, but cross-reactivity with non-target proteins (e.g., other F-box family members) should be confirmed experimentally.

• Experimental Design: Optimal performance is achieved when paired with protocols involving denaturing gels (e.g., SDS-PAGE) and immunoblotting .

• Sample Preparation: For IP, solubilization of membrane-associated F-box proteins may require detergents like Triton X-100 or RIPA buffer .

Comparative Analysis with Other Plant Antibodies

The At5g52880 Antibody shares functional similarities with other plant-specific antibodies but distinguishes itself in targeting F-box proteins. Below is a comparison with related antibodies:

Note: While the PRMT5 Antibody is used in human autoimmune studies, it highlights the broader utility of antibody-based approaches in protein interaction studies.

Future Directions and Unexplored Avenues

• Epitope Mapping: Determining the exact binding sites of the At5g52880 Antibody could enhance its utility in structural and functional studies.

• Therapeutic Potential: F-box proteins are implicated in plant stress responses; insights from antibody-mediated studies may inform strategies to engineer disease-resistant crops.

• High-Throughput Screening: Integration with CRISPR-Cas9 systems could enable genome-wide screens to identify F-box protein substrates.