At5g62510 Antibody

Functional Context of the Target Protein

The At5g62510 gene encodes an F-box protein, a component of the SCF (SKP1-CUL1-F-box) ubiquitin ligase complex. These complexes mediate substrate-specific protein degradation, a critical regulatory mechanism in plant development and stress responses . Key roles include:

• Substrate Recognition: F-box proteins bind specific substrates for ubiquitination.

• Stress Signaling: Linked to pathways activated under environmental stressors.

• Developmental Regulation: Influences cell cycle progression and hormone signaling.

No direct functional studies of the At5g62510 gene product were identified in the provided sources, highlighting a gap in published research .

Antibody Validation and Usage

While validation data (e.g., immunoblot images, specificity tests) are not explicitly provided in the sources, commercial antibodies like CSB-PA884263XA01DOA are typically validated for:

• Specificity: Immunogen-derived affinity confirmed via knockout/knockdown controls.

• Titer: Concentration optimized for common assays.

• Cross-Reactivity: Assumed specificity for Arabidopsis thaliana unless stated otherwise.

For experimental reliability, users are advised to validate the antibody in their specific model system .

Comparative Analysis of Related Antibodies

The table below contextualizes At5g62510 Antibody within a subset of Arabidopsis F-box protein-targeting reagents :

This comparative framework aids in selecting antibodies for pathway-specific studies.

Research Implications and Gaps

• Potential Applications:

  • Mechanistic studies of ubiquitin-proteasome systems in plants.

  • Phenotypic analysis of At5g62510 knockout mutants.

• Limitations:

  • No peer-reviewed studies directly investigating At5g62510 were found in the provided sources.

  • Structural and interaction data (e.g., crystallography, yeast two-hybrid) are absent.