At2g29610 Antibody

Overview of At2g29610 Antibody

The At2g29610 Antibody is a research tool designed to target the Arabidopsis thaliana protein encoded by the gene At2g29610. This gene is annotated as a pseudogene belonging to the F-box protein family, which typically mediates protein degradation via the ubiquitin-proteasome system . The antibody is part of a broader panel of Arabidopsis-specific antibodies, as evidenced by its inclusion in commercial catalogs alongside other gene-specific reagents .

Role in Protein Localization Studies

The At2g29610 Antibody has been implicated in studies examining the endosperm localization of MIPS1, MIPS2, and MIPS3 proteins in Arabidopsis . While the exact mechanism of At2g29610 in this context remains unclear, its association with F-box proteins suggests a potential role in regulating protein stability or degradation.

Functional Analysis in Meiosis

Recent studies investigated the role of F-box genes, including At2g29610, in meiotic recombination . Researchers tested T-DNA mutants for several F-box genes (e.g., At4g29420, At2g29610, At3g10430) to assess their impact on fertility and meiotic processes. Key findings include:

• No Defect in Single Mutants: At2g29610 mutants did not exhibit fertility defects or meiotic abnormalities, suggesting functional redundancy or a minor role in meiosis .

• Potential Redundancy: The lack of phenotypic changes in single mutants implies that At2g29610 may act redundantly with other F-box proteins or require combinatorial inactivation for observable effects .

Limitations in Current Research

Despite its inclusion in F-box gene studies, detailed biochemical or functional data (e.g., epitope specificity, binding affinity, or cross-reactivity) for the At2g29610 Antibody remain unpublished. Its role in meiotic pathways or protein degradation complexes is inferred from gene family associations rather than direct experimental evidence .

Comparative Analysis of F-Box Genes in Meiosis

The table below summarizes F-box genes tested for meiotic roles, including At2g29610 :

Future Directions

1. Epitope Mapping: Characterization of the antibody’s binding specificity to validate its utility in detecting At2g29610 or related pseudogene products.

2. Functional Redundancy: Investigation of combinatorial mutants (e.g., At2g29610 + At3g61730) to uncover synergistic roles in meiosis.

3. Protein Interactome: Identification of At2g29610-associated proteins to clarify its biological function.

References

1. Commercial Catalog: Antibody product specifications from CUSABIO .

2. Pseudogene Annotation: Association with F-box proteins and MIPS localization studies .

3. Meiotic Gene Analysis: Phenotypic data from F-box gene mutant screens .