At2g38590 Antibody

Introduction

At2g38590 Antibody is a specialized research reagent designed to detect and study the protein encoded by the AT2G38590 gene in Arabidopsis thaliana (thale cress). This antibody is primarily used in molecular biology and plant sciences to investigate the function of the protein it targets, which belongs to the F-box and associated interaction domains-containing protein family. Below is a detailed analysis of the gene, antibody characteristics, and potential research applications, synthesized from available literature and product specifications.

Gene Overview: AT2G38590

Gene Symbol: AT2G38590
Full Name: F-box and associated interaction domains-containing protein
Synonyms: T6A23.21, T6A23_21
Organism: Arabidopsis thaliana

The AT2G38590 gene encodes a protein with structural features characteristic of F-box proteins, which are typically involved in protein degradation pathways via the ubiquitin-proteasome system. F-box proteins often serve as substrate receptors in E3 ubiquitin ligase complexes, targeting specific proteins for ubiquitination and subsequent degradation. While the exact biological role of the AT2G38590-encoded protein remains uncharacterized in the provided literature, its classification suggests potential involvement in regulating protein stability or signaling pathways in plants.

Antibody Product Details

The At2g38590 Antibody is commercially available as a research-grade reagent. Key specifications include:

Applications:

• Western blotting

• Immunoprecipitation

• Immunohistochemistry (IF/IHC)

• ELISA

Note: Specific experimental validation data (e.g., cross-reactivity, epitope mapping) are not publicly disclosed in the provided sources.

Protein Degradation Pathways

Given the F-box domain, the AT2G38590 protein may interact with E3 ligase complexes (e.g., SCF complexes) to regulate protein turnover. The antibody could be used to:

• Identify substrates of the AT2G38590 protein.

• Study stress responses (e.g., drought, pathogen infection) in plants, where protein degradation is critical.

Functional Genomics

• Knockout/Overexpression Studies: Validate gene function in Arabidopsis mutants.

• Protein Localization: Determine subcellular localization (e.g., cytoplasm, nucleus) via immunofluorescence.

Comparative Proteomics

• Analyze AT2G38590 expression across plant tissues (e.g., roots, leaves, flowers).

• Compare expression levels in wild-type vs. mutant strains.

Challenges and Limitations

1. Lack of Validation Data: No peer-reviewed studies explicitly report the use of the At2g38590 Antibody in experiments.

2. Cross-Reactivity Concerns: F-box proteins often share conserved domains, raising potential for off-target binding.

3. Species Specificity: Primarily designed for Arabidopsis, limiting utility in other organisms.