At3g22700 Antibody
Description
Primary Techniques
-
ELISA: Used to quantify At3g22700 protein levels in lysates or extracts.
-
Western Blot (WB): Detects the presence and size of the target protein in samples.
Potential Research Focus Areas
-
Protein Expression Analysis:
-
Studying developmental or stress-induced changes in At3g22700 expression.
-
Investigating tissue-specific localization (e.g., roots, leaves).
-
-
Protein Interaction Studies:
-
Identifying binding partners via co-immunoprecipitation (IP) followed by WB.
-
-
Functional Characterization:
-
Knockout or overexpression studies to infer At3g22700’s role in cellular processes.
-
Comparative Analysis with Other Antibodies
| Feature | At3g22700 Antibody | General Monoclonal Antibodies | General Polyclonal Antibodies |
|---|---|---|---|
| Specificity | Broad epitope recognition | Single epitope recognition | Multiple epitope recognition |
| Cross-Reactivity | Limited (species-specific) | High (if engineered) | Variable (depends on immunogen) |
| Production Time | Made-to-order (14–16 weeks) | Faster (pre-made clones) | Longer (polyclonal generation) |
| Use Cases | ELISA, WB | High-throughput assays, diagnostics | Complex antigen detection |
Published Data
-
ELISA: Sensitive quantification of protein levels.
-
WB: Confirmation of protein size and presence in complex mixtures.
Potential Challenges
-
Cross-Reactivity: Polyclonal antibodies may bind non-specific epitopes, requiring validation.
-
Limited Availability: Custom production (14–16 weeks) may delay experimental timelines.
Optimal Experimental Design
-
Sample Preparation:
-
Lyse plant tissues using protocols compatible with WB (e.g., RIPA buffer).
-
Clarify lysates to reduce non-specific binding.
-
-
Antibody Dilution:
-
Follow supplier guidelines (e.g., 1:1000–1:3000 for WB).
-
Optimize dilution based on signal-to-noise ratio.
-
-
Controls:
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Q&A
Based on the provided search results and analysis of available scientific literature, there is no direct evidence of an antibody targeting the Arabidopsis thaliana AT3G22700 gene product (an F-box protein). This gene is associated with plant-specific regulatory functions, and the search results do not reference antibody development or applications for this target. Below are research-focused FAQs addressing common scenarios where this type of inquiry might arise, synthesized from adjacent antibody research methodologies in the provided sources.
Advanced Research Questions
-
How can design of experiments (DOE) principles improve antibody validation workflows?
-
Case study framework:
-
Statistical analysis: Use fractional factorial designs to identify critical parameters while minimizing experimental runs .
-
-
How do researchers resolve discrepancies in antibody performance across plant tissues?
-
Troubleshooting workflow:
-
Confirm tissue-specific post-translational modifications (e.g., glycosylation) via glycan profiling .
-
Compare antibody performance in transgenic lines overexpressing AT3G22700 versus wild-type controls .
-
Validate with orthogonal methods (e.g., CRISPR-Cas9 tagging followed by fluorescence microscopy) .
-
-
Data Contradiction Analysis
-
What steps are taken when an antibody shows inconsistent binding across replicates?
-
Root-cause investigation:
-
