At3g27290 Antibody
Description
Introduction to At3g27290 Antibody
The At3g27290 Antibody is a polyclonal antibody specific to the Arabidopsis thaliana protein encoded by the AT3G27290 gene. This antibody targets a recombinant protein derived from this gene and is primarily used in research applications such as immunoblotting (WB) and enzyme-linked immunosorbent assays (ELISA). It is raised in rabbits, ensuring cross-reactivity with Arabidopsis thaliana proteins, and is stored under cryogenic conditions to maintain stability .
3.1. Protein Expression Analysis
The antibody is optimized for detecting At3g27290 protein expression levels in Arabidopsis tissues. In Western blotting, it enables precise identification of protein bands corresponding to the target antigen, critical for studying gene expression under varying environmental or developmental conditions .
3.2. Subcellular Localization
While not explicitly tested in peer-reviewed studies, the antibody’s compatibility with ELISA and WB suggests potential utility in immunocytochemistry or immunohistochemistry to map At3g27290 localization within plant cells.
3.3. Functional Studies
Research on analogous antibodies in plant biology (e.g., iron uptake studies in barley ) highlights the role of such reagents in probing protein interactions, post-translational modifications, or enzymatic activity. The At3g27290 Antibody may facilitate similar investigations into the gene’s biological role in Arabidopsis.
5.1. Role of Antibodies in Plant Molecular Biology
Antibodies like At3g27290 are indispensable for studying gene function in model organisms. For example, immunoblotting with species-specific antibodies has been pivotal in elucidating iron uptake mechanisms in plants , demonstrating the utility of such tools in dissecting metabolic pathways.
5.2. Challenges and Future Directions
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Specificity: Polyclonal antibodies may exhibit off-target binding, necessitating validation via blocking assays or immunoprecipitation.
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Scalability: Long lead times (14–16 weeks) for production may limit high-throughput studies.
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Therapeutic Potential: While not designed for clinical use, insights from antibody engineering (e.g., pH-dependent binding ) could inspire future applications in agricultural biotechnology.
Product Specs
Q&A
The provided search results do not contain any information related to "At3g27290 Antibody" or Arabidopsis thaliana gene AT3G27290. All referenced studies focus on antibodies targeting:
This discrepancy suggests either:
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A potential typographical error in the gene identifier
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An emerging research target not yet represented in indexed literature
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Context-specific nomenclature differences between plant and biomedical research
For Arabidopsis researchers studying AT3G27290 (a pentatricopeptide repeat-containing protein involved in RNA editing), I recommend:
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Validating antibody specificity through:
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Considering epitope tagging approaches given PPR protein complexity
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Cross-referencing with TAIR (The Arabidopsis Information Resource) for updated annotations
