At4g22060 Antibody

Introduction to At4g22060 Antibody

At4g22060 Antibody is a polyclonal antibody specifically designed to recognize and bind to the At4g22060 protein from Arabidopsis thaliana, commonly known as mouse-ear cress or thale cress. This antibody represents a significant tool for researchers studying plant molecular biology, particularly those focusing on protein-protein interactions, ubiquitination pathways, and F-box protein functions in Arabidopsis .

Developed through immunization of rabbits with recombinant Arabidopsis thaliana At4g22060 protein, this antibody demonstrates high specificity for its target antigen. The polyclonal nature of the antibody ensures recognition of multiple epitopes on the target protein, enhancing detection sensitivity across various experimental applications .

Structure and Properties of At4g22060 Antibody

At4g22060 Antibody belongs to the Immunoglobulin G (IgG) class of antibodies. As a polyclonal antibody, it consists of a heterogeneous mixture of antibodies produced by different B-cell lineages in response to immunization with the recombinant At4g22060 protein . This heterogeneity contributes to its ability to recognize different epitopes on the target protein.

The antibody is supplied in liquid form, preserved in a buffer solution containing 0.03% Proclin 300 as a preservative, 50% Glycerol for stability, and 0.01M PBS at pH 7.4 to maintain optimal conditions for antibody integrity . These storage conditions are critical for preserving the antibody's binding capacity and specificity over time.

Target Antigen: At4g22060 Protein

The target of At4g22060 Antibody is the protein encoded by the At4g22060 gene from Arabidopsis thaliana. This protein belongs to the F-box family and contains a domain of unknown function (DUF295) . F-box proteins are known to play crucial roles in protein-protein interactions and ubiquitin-mediated protein degradation pathways in plants.

The At4g22060 protein, also known by its alias AtFDB29, is characterized as a probable F-box protein . F-box proteins typically function as part of the SCF (Skp1, Cullin, F-box) ubiquitin ligase complex, which is involved in targeting proteins for ubiquitination and subsequent degradation by the 26S proteasome. This process is central to numerous cellular processes including hormone signaling, development, and stress responses in plants.

Table 2: Properties of At4g22060 Target Protein

Applications and Uses of At4g22060 Antibody

At4g22060 Antibody has been validated for several experimental applications, making it a versatile tool for researchers investigating plant molecular biology and protein function . The primary applications include:

Enzyme-Linked Immunosorbent Assay (ELISA)

The antibody has been validated for use in ELISA, allowing researchers to detect and quantify At4g22060 protein in complex biological samples. This application is particularly useful for studying protein expression levels under various experimental conditions or developmental stages .

Western Blotting (WB)

At4g22060 Antibody has been tested for Western blotting applications, enabling the detection of the target protein in cell or tissue lysates. This technique allows researchers to determine the molecular weight of the protein and assess its expression levels in different samples .

Immunohistochemistry and Immunocytochemistry

While specific validation data is not provided in the search results, polyclonal antibodies of this nature are often suitable for immunohistochemical and immunocytochemical applications, allowing researchers to visualize the subcellular localization of At4g22060 protein in plant tissues or cells.

Handling Recommendations

When working with At4g22060 Antibody, it is recommended to aliquot the stock solution to minimize freeze-thaw cycles. For applications such as Western blotting and ELISA, appropriate dilution factors should be determined empirically for optimal results.

Research and Scientific Applications

While specific research findings utilizing At4g22060 Antibody are not extensively documented in the provided search results, the antibody represents a valuable tool for investigating several aspects of plant molecular biology:

F-box Protein Function Studies

As At4g22060 is classified as an F-box protein, the antibody enables researchers to study its role in protein-protein interactions and ubiquitin-mediated protein degradation pathways in Arabidopsis thaliana. These studies can provide insights into how this specific F-box protein contributes to plant development, stress responses, or hormone signaling .

DUF295 Domain Characterization

The At4g22060 protein contains a domain of unknown function (DUF295), and the antibody facilitates studies aimed at characterizing this domain's structure and function. Such research may reveal novel mechanisms or pathways in plant cellular processes.

Comparative Studies with Related F-box Proteins

The antibody can be used in comparative studies examining the expression, localization, or function of At4g22060 alongside other F-box proteins in Arabidopsis, such as those listed in search result , including AT1G67160, AT4G22165, AT4G17565, and others. These studies can help elucidate the specific roles of individual F-box proteins within the broader context of the plant ubiquitination machinery.