At4g22165 Antibody
Description
AT4G22165 Gene Overview
AT4G22165 is annotated as a probable F-box protein involved in protein degradation pathways, though its precise biological function remains uncharacterized . Key details include:
| Property | Value |
|---|---|
| Locus ID | 505006507 |
| TAIR Short Description | F-box family protein with DUF295 domain |
| Aliases | AtFDB30 |
| Protein Family | F-box (SCF complex component) |
| Expression Patterns | Predicted based on computational models (no experimental data cited) |
This gene is part of the broader F-box protein family, which regulates ubiquitination and proteasomal degradation of target proteins in plants .
Recombinant Protein Availability
Commercial sources offer recombinant AT4G22165 protein expressed in E. coli, yeast, baculovirus, or mammalian systems . These products could theoretically be used to generate anti-AT4G22165 antibodies for research purposes, though no such antibodies are explicitly documented in literature.
| Recombinant Source | Expression System | Applications |
|---|---|---|
| CSB-YP316739DOA | Yeast | Western blot, ELISA, antibody production |
| CSB-EP316739DOA | E. coli | Structural studies, affinity assays |
| CSB-MP316739DOA | Mammalian cells | Functional assays (e.g., ubiquitination studies) |
Hypothetical Antibody Applications
While no specific antibodies targeting AT4G22165 are reported, general antibody strategies applicable to F-box proteins include:
Functional Studies
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Ubiquitination assays: Antibodies could detect AT4G22165 interactions with E3 ligase complexes or substrate proteins .
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Protein localization: Fluorescently tagged antibodies might map AT4G22165 to cellular compartments (e.g., cytoplasm, nucleus).
Diagnostic/Therapeutic Potential
F-box proteins are often targets in plant stress responses. Antibodies could:
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Monitor protein levels: Quantify AT4G22165 expression under abiotic stress.
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Inhibit degradation pathways: Block SCF complex activity to study downstream phenotypes.
Challenges in Antibody Development
Development of anti-AT4G22165 antibodies faces hurdles common to plant protein immunology:
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Low immunogenicity: DUF295 domains may lack conserved epitopes, necessitating peptide-based immunization.
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Cross-reactivity: F-box proteins share structural motifs, increasing off-target binding risks.
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Validation complexity: Requires orthogonal methods (e.g., CRISPR knockout) to confirm specificity.
Related Antibody Research in Plant Biology
Though not directly linked to AT4G22165, antibody-based tools in plant science include:
Product Specs
Components: 50% Glycerol, 0.01M PBS, pH 7.4
Q&A
Based on a comprehensive analysis of current antibody research methodologies and principles from peer-reviewed studies, here are FAQs tailored for academic researchers working with specialized antibodies like At4g22165:
Advanced Research Questions
How to resolve contradictory binding affinity data across assays?
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Case example from IL-4Rα antibody development :
Assay KD (nM) Notes BLI (monomeric) 1.04 Gold standard for intrinsic affinity SPR (bivalent) 0.01 Shows avidity effects
How to establish reliable negative controls for plant studies?
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Recommended control matrix:
What statistical approaches handle batch effects in antibody-based screens?
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Apply linear mixed-effects model:
Where:-
: Antibody lot random effect
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: Plant growth chamber fixed effect
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Emerging Techniques
How to integrate SERS-based detection for low-abundance targets?
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Protocol adapted from influenza NA detection :
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Conjugate antibodies to 60nm AuNPs (OD520 = 3-4)
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Use Raman reporter molecule: 4-mercaptobenzoic acid (10μM)
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Validate with spike-recovery experiments (70-130% acceptable range)
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