At1g54550 Antibody

Introduction to At1g54550 Antibody

The At1g54550 antibody is a specialized reagent designed to target the protein encoded by the AT1G54550 gene in Arabidopsis thaliana. This gene is annotated as a "F-box and associated interaction domains-containing protein", part of the F-box protein family involved in substrate recognition for ubiquitin-mediated proteolysis . Antibodies against such proteins are critical tools for studying plant biology, particularly in understanding protein degradation pathways and regulatory mechanisms.

Gene and Protein Structure

• Gene ID: AT1G54550 (Arabidopsis Genome Initiative)

• Protein: F-box/RNI-like superfamily protein with domains implicated in protein-protein interactions .

• Function: F-box proteins typically form part of SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complexes, marking specific substrates for proteasomal degradation .

Antibody Development

While specific details about the At1g54550 antibody’s immunogen or epitope are not publicly available in the provided sources, polyclonal antibodies targeting plant F-box proteins are generally raised against synthetic peptides or recombinant protein fragments . These antibodies are validated for applications such as:

• Western blotting

• Immunohistochemistry

• Subcellular localization studies

Role in Plant Physiology

F-box proteins like AT1G54550 regulate diverse processes, including:

• Hormone signaling (e.g., auxin, jasmonate)

• Stress responses

• Developmental transitions

Antibodies enable researchers to track protein expression levels and localization under varying conditions (e.g., pathogen exposure, abiotic stress) .

Interaction Studies

Co-immunoprecipitation (Co-IP) using the At1g54550 antibody could identify binding partners, elucidating its role in SCF complexes .

Challenges and Limitations

• Specificity: Cross-reactivity with homologous F-box proteins (e.g., MIPS family) is a potential concern .

• Data Availability: Public databases and literature lack detailed characterization of this antibody, indicating it may be a niche research tool .

Comparative Analysis of F-box Protein Antibodies

Future Directions

• Epitope Mapping: Clarify the antibody’s binding region to improve specificity .

• Functional Studies: Investigate AT1G54550’s role in stress adaptation using knockout mutants and antibody-based assays .