At1g57580 Antibody
Description
Introduction to At1g57580
At1g57580 is a gene in Arabidopsis thaliana encoding an F-box family protein, as annotated in Araport11 . F-box proteins are critical components of the Skp1-Cullin-F-box (SCF) ubiquitin ligase complexes, which mediate protein degradation via the ubiquitin-proteasome system. These proteins play roles in plant development, stress responses, and hormonal signaling.
At1g57580 Antibody: Definition and Purpose
The "At1g57580 antibody" refers to a polyclonal or monoclonal antibody designed to detect the protein product of the At1g57580 gene. Such antibodies are typically used in:
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Western blotting to confirm protein expression levels.
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Immunolocalization to determine subcellular protein distribution.
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Functional studies to investigate interactions with other proteins or substrates.
Related Antibody Research in Plant Systems
While At1g57580-specific antibodies are not well-documented, studies on analogous plant antibodies provide insights into potential applications:
Key Findings from Arabidopsis Antibody Studies
These examples highlight the utility of antibodies in elucidating plant protein functions, though At1g57580 itself remains understudied .
Challenges in Antibody Validation
Commercial antibodies for plant proteins often lack rigorous validation, as seen in studies on angiotensin II receptor antibodies . For At1g57580, critical validation steps would include:
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Specificity testing: Western blotting against knockout mutants to confirm absence of cross-reactivity.
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Epitope mapping: Ensuring the antibody targets a unique region of the At1g57580 protein.
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Functional assays: Demonstrating antibody utility in co-immunoprecipitation or enzyme-linked immunosorbent assays (ELISAs).
Future Directions
To advance research on At1g57580, the following steps are recommended:
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Antibody production: Use peptide antigens derived from the At1g57580 protein sequence for immunization.
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Enhanced validation: Apply protocols from studies like to avoid non-specific binding.
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Integration with omics data: Correlate antibody-based protein detection with transcriptomic or proteomic datasets.
Product Specs
Q&A
The provided search results focus on angiotensin II type 1 receptor (AT1R) antibodies rather than Arabidopsis thaliana gene At1g57580. Assuming this discrepancy stems from a typographical error, the following FAQs address AT1R antibody research in alignment with the academic rigor and methodological focus requested.
Advanced Research Questions
How can functional assays resolve AT1R antibody mechanisms in disease models?
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Dynamic mass redistribution (DMR): Measures whole-cell morphological changes in AT1R-transfected HEK293 cells. Example: A monoclonal AT1R antibody amplified angiotensin II (Ang II) responses without standalone activity .
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Electrophysiological neutralization: Assess synaptotoxicity rescue in brain extracts using antibodies like 71A1 .
What in vivo models elucidate AT1R antibody-induced pathologies?
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C57BL/6J mice: Immunization with membrane-embedded AT1R caused interstitial lung disease (ILD) and dermal fibrosis, dependent on CD4+ T cells and B cells .
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Adoptive transfer models: Transferring monoclonal AT1R antibodies into knockout mice confirmed receptor-specific effects (e.g., reduced inflammation in AT1Ra/b-/- mice) .
How should researchers address contradictory clinical correlations of AT1R autoantibodies?
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Threshold-based analysis: In COVID-19, AT1R antibody positivity (≥10 U/mL) correlated with milder disease (14.86% vs. 29.46% in controls), conflicting with prior literature .
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Cohort stratification: Subdivide patients by antibody titers and comorbidities to isolate confounding variables .
Data Table: Key Findings on AT1R Antibody Specificity
Methodological Recommendations
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For functional studies: Use nanobody antagonists (e.g., maternally selective heavy chain-only antibodies) to achieve receptor-specific modulation without cross-reactivity .
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For clinical correlations: Combine ELISA with functional assays (e.g., DMR) to distinguish pathogenic from benign autoantibodies .
