At1g12855 Antibody
Description
Antibody Development and Availability
The At1g12855 antibody is produced using recombinant protein fragments as immunogens. Commercial sources provide variants optimized for different applications:
| Product Code | Expression System | Conjugate | Applications |
|---|---|---|---|
| CSB-YP881774DOA | Yeast | None | WB, ELISA |
| CSB-EP881774DOA | E. coli | None | Immunoprecipitation, IHC |
| CSB-EP881774DOA-B | E. coli | Biotin (AviTag) | Flow cytometry, diagnostics |
| CSB-BP881774DOA | Baculovirus | None | Structural studies |
| CSB-MP881774DOA | Mammalian cells | None | Functional assays |
Source: Cusabio product listings .
Protein Degradation Studies
The antibody enables tracking of At1g12855 in SCF complexes, particularly in plant stress responses. For example:
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Ubiquitination assays: Identifies substrates marked for proteasomal degradation .
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Subcellular localization: Confirms nuclear or cytoplasmic distribution via immunofluorescence .
Genetic Interaction Mapping
Used in co-immunoprecipitation (Co-IP) to identify binding partners such as:
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ASK proteins (Arabidopsis Skp1 homologs)
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Cullin scaffolds
Validation and Limitations
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Specificity: Validated against recombinant At1g12855 in Western blots (WB) with <5% cross-reactivity to related F-box proteins .
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Sensitivity: Detects ≥10 ng of target protein in chemiluminescent assays .
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Challenges: No peer-reviewed studies directly using this antibody were identified in the literature, highlighting a gap in functional characterization .
Future Directions
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CRISPR knockouts: Pairing with mutant lines to assess phenotypic impacts.
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Phosphorylation studies: Investigating post-translational regulation under abiotic stress.
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Therapeutic exploration: Engineered variants (e.g., Fc fusions) could modulate plant immune pathways, inspired by GPCR-targeting nanobodies .
Product Specs
Components: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
