At2g02240 Antibody

Introduction to At2g02240 Antibody

The At2g02240 Antibody (Product Code: CSB-PA666873XA01DOA) is a polyclonal rabbit antibody designed for use in immunological assays such as ELISA and Western blotting (WB) . It specifically recognizes the recombinant At2g02240 protein, which is annotated in the Arabidopsis thaliana genome as a member of the F-box family of proteins . F-box proteins are critical components of E3 ubiquitin ligase complexes, facilitating the ubiquitination and degradation of target proteins .

Functional Insights into At2g02240

The AT2G02240 gene encodes a protein with a conserved F-box motif, a hallmark of F-box proteins that interact with Skp1/Cdc53/F-box (SCF) complexes . These complexes mediate the ubiquitination of substrate proteins, targeting them for degradation via the 26S proteasome. While the exact substrate of At2g02240 remains uncharacterized, F-box proteins in Arabidopsis are known to regulate diverse processes, including:

• Stress responses: Hormone signaling (e.g., auxin, gibberellin).

• Developmental regulation: Flowering time, cell differentiation.

• Protein quality control: Degradation of misfolded or regulatory proteins.

Experimental Validation

The antibody has been validated for use in ELISA and Western blotting to detect At2g02240 in Arabidopsis tissues . No cross-reactivity data with other species or paralogs is currently available.

Potential Research Applications

1. Protein Localization:

   • Study subcellular distribution of At2g02240 in plant tissues.

   • Investigate interactions with SCF complexes via co-immunoprecipitation (IP).

2. Functional Studies:

   • Monitor protein abundance under stress conditions (e.g., drought, salt).

   • Analyze phenotypic changes in AT2G02240 knockout or overexpression mutants.

3. Biomarker Discovery:

   • Identify At2g02240 as a marker for specific developmental stages or stress responses.

Research Considerations

1. Limitations:

   • Restricted to Arabidopsis thaliana; no validated cross-reactivity with other species.

   • Requires specialized protocols for plant protein extraction.

2. Best Practices:

   • Optimize antibody dilution (e.g., 1:1000–1:5000 for WB) based on sample type.

   • Use β-actin or tubulin as loading controls in WB experiments.