At4g13985 Antibody

Introduction to At4g13985 Antibody

The At4g13985 Antibody is a specialized immunoglobulin targeting the protein product of the At4g13985 gene in Arabidopsis thaliana (mouse-ear cress). This gene encodes an FBD-associated F-box protein (FBD1), part of the ubiquitin-proteasome system involved in targeted protein degradation . The antibody is utilized to study FBD1's role in cellular processes, including stress responses, developmental regulation, and protein turnover.

Target Protein Overview

• Gene ID: AT4G13985 (Arabidopsis thaliana)

• Protein: FBD1 (F-box domain-containing protein)

• Function: Mediates substrate recognition for ubiquitination, facilitating proteasomal degradation .

Functional Studies

• Overexpression Analysis: Studies indicate that FBD1 overexpression alters protein degradation pathways, affecting stress tolerance and flowering time .

• Substrate Interaction: FBD1 interacts with SKP1-like proteins, forming SCF complexes essential for ubiquitin ligase activity .

Experimental Data

Data from analogous Arabidopsis antibodies (e.g., At4g13960, At3g58920) reveal technical benchmarks :

Challenges and Limitations

• Specificity Issues: Cross-reactivity with homologous F-box proteins (e.g., At3g49150) may occur due to shared domains .

• Commercial Availability: As of 2025, no vendor explicitly lists At4g13985 antibodies, necessitating custom production .

Future Directions

• Structural Characterization: Cryo-EM or X-ray crystallography to map epitope-antibody interactions.

• CRISPR Knockout Models: Validate antibody specificity using At4g13985-null mutants.