At1g55030 Antibody

Research Applications

The At1g55030 antibody is part of Abmart’s AbInsure™ program, which guarantees performance in specified applications :

• Western Blot: Validated for detecting endogenous At1g55030 protein in Arabidopsis lysates.

• Immunoprecipitation (IP): Recommended for isolating At1g55030 complexes.

• Functional Studies: Investigates roles in ubiquitination pathways, given F-box proteins’ involvement in substrate recognition for E3 ligases.

Validation and Specificity

• Epitope Mapping: Targets linear epitopes in the N-terminal region (exact sequences disclosed upon shipment).

• Cross-Reactivity: No reported cross-reactivity with other Arabidopsis F-box proteins .

• Performance: Validated using immunogen peptides, with dot blot confirming specificity.

Limitations and Research Gaps

While the antibody is commercially available, peer-reviewed studies directly utilizing it are sparse. Current knowledge relies on manufacturer data, highlighting a need for independent validation. For example:

• No publications in PubMed or PMC cite At1g55030 antibody use as of March 2025.

• Functional studies linking At1g55030 to specific biochemical pathways remain unpublished.

Future Directions

• Mechanistic Studies: Elucidate At1g55030’s role in ubiquitin-mediated protein degradation.

• Comparative Analyses: Compare expression across Arabidopsis mutants or stress conditions.

• Structural Biology: Solve crystal structures of antibody-antigen complexes to refine epitope mapping.