At1g05080 Antibody

Biological Context and Target Specification

The At1g05080 Antibody specifically targets the protein product of the At1g05080 gene, a locus in the Arabidopsis thaliana genome with the UniProt identifier Q9ZVP2. While the exact biological function of this protein remains under investigation, its study contributes to broader understanding of plant cellular processes.

Primary research applications:

1. Protein localization: Subcellular tracking via immunofluorescence or immunogold labeling.

2. Expression profiling: Western blot analysis across developmental stages or stress conditions.

3. Interaction studies: Co-immunoprecipitation to identify binding partners.

Current limitations:

• No peer-reviewed publications specifically citing this antibody were identified in available databases ( ).

• Functional characterization of the target protein remains incomplete, limiting interpretative scope.

Technical Considerations for Experimental Use

Optimal performance requires attention to:

Experimental parameters:

• Sample preparation: Use plant-specific extraction buffers to preserve epitope integrity.

• Controls: Include Arabidopsis knockout lines (if available) to confirm antibody specificity.

• Cross-reactivity: Screen against related Arabidopsis proteins due to potential conserved domains.

Validation metrics (recommended):

1. Signal disappearance in CRISPR-Cas9 knockout mutants

2. Band size correspondence with predicted molecular weight (~50 kDa, based on gene sequence)

Future Research Directions

Critical knowledge gaps to address:

1. Functional annotation: High-throughput phenotyping of At1g05080 mutant plants.

2. Antibody validation: Publication of specificity tests using orthogonal methods (e.g., mass spectrometry).

3. Translational potential: Investigation of homologs in crop species for agricultural applications.