At5g38565 Antibody

Antibody Development and Validation

At5g38565 antibodies are mouse monoclonal antibodies generated against synthetic peptides representing the N-terminal, C-terminal, and mid-region (M-terminal) sequences of the protein. Key validation metrics include:

Recombinant At5g38565 protein (produced in yeast, E. coli, baculovirus, or mammalian cells) is available for antibody validation and functional studies .

Research Applications

Validated applications for At5g38565 antibodies include:

• Western Blot (WB)

• Immunofluorescence (IF)

• Immunohistochemistry (IHC)

• Immunoprecipitation (IP)/Chromatin IP (ChIP)

These antibodies are optimized for detecting endogenous At5g38565 in Arabidopsis thaliana samples .

Functional Significance

As an F-box protein, At5g38565 is implicated in:

• Ubiquitin-mediated proteolysis: F-box proteins typically recruit substrates for E3 ubiquitin ligase complexes.

• Plant development: FBD-associated domains often interact with conserved regulatory pathways.

While structural studies of the antibody-antigen interaction are not publicly available, computational modeling tools like Rosetta Antibody could predict binding dynamics .

Limitations and Alternatives

• Species specificity: No cross-reactivity data with non-Arabidopsis species are reported.

• Custom solutions: Abmart offers tailored antibody development for unique requirements (e.g., epitope specificity, cross-species reactivity) .