At1g66310 Antibody
Description
AT1 Receptor Antibodies
If "At1g66310" was intended to denote Angiotensin II Type 1 (AT1) receptor antibodies, the following findings are relevant:
Critical Insights:
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Specificity Challenges: Multiple commercial AT1 receptor antibodies (e.g., sc-1173, AAR-011) show non-specific binding, as they detect identical bands in wild-type and knockout mice .
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Clinical Relevance: Anti-AT1R antibodies are linked to systemic sclerosis (SSc) but do not predict disease severity or organ involvement .
2.2 Beta-Actin Antibodies
If "At1g66310" refers to beta-actin antibodies, the following data applies:
| Antibody (66009-1-Ig) | Reactivity | Applications | Dilution Recommendations |
|---|---|---|---|
| Mouse IgG2b | Human, mouse, rat, pig, rabbit, chicken | WB, IHC, IF, IP, FC | WB: 1:20,000–1:100,000; IHC: 1:20–1:2,000 |
Key Findings:
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Utility: Beta-actin antibodies are used as loading controls in Western blots due to their constitutive expression .
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Limitations: Expression variability in tissues and developmental stages may affect normalization accuracy .
3. Antibody Structure and Mechanism
While no data exists for "At1g66310 Antibody," general antibody biology principles apply:
Antibody Structure
Post-Translational Modifications (PTMs)
Antibodies may undergo PTMs (e.g., glycosylation, phosphorylation) to enhance stability or binding affinity. For example, beta-actin antibodies are influenced by acetylation and phosphorylation .
Research Gaps and Recommendations
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Clarification of Nomenclature: Verify the correct identifier for the antibody (e.g., gene symbol, catalog number).
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Target Validation: Confirm the antigen (e.g., AT1 receptor, beta-actin) and ensure antibody specificity via knockout controls .
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Functional Testing: Evaluate antibody efficacy using bioassays (e.g., luminometric assays for AT1R antibodies) .
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
