At1g66320 Antibody
Description
Basic Characteristics
The At1g66320 Antibody is designed for immunological detection of the protein encoded by the At1g66320 gene in Arabidopsis thaliana. Key details include:
| Property | Value |
|---|---|
| Product Code | CSB-PA880215XA01DOA |
| Uniprot ID | Q9C8Y5 |
| Species | Arabidopsis thaliana |
| Format | Liquid (presumed, based on similar antibodies) |
| Concentration | 2ml or 0.1ml (standard for monoclonal antibodies) |
Source: Cusabio product listing
Potential Applications
While no direct experimental data exists for this antibody, its design suggests compatibility with standard immunodetection techniques. Common applications for plant-specific antibodies include:
| Technique | Purpose |
|---|---|
| Western Blot (WB) | Detection of At1g66320 protein in lysates or purified fractions |
| Immunohistochemistry (IHC) | Localization of At1g66320 in plant tissues |
| Immunoprecipitation (IP) | Isolation of At1g66320-containing protein complexes |
| Immunofluorescence (IF) | Cellular localization in fixed or live plant cells |
General antibody applications inferred from beta-actin antibody data and AT1 receptor antibody validation studies .
Antibody Validation Considerations
Critical validation steps for novel antibodies include:
| Criterion | Method |
|---|---|
| Specificity | Western blotting with knockout Arabidopsis lines (if available) |
| Cross-reactivity | Testing against related proteins (e.g., homologs in Brassica spp.) |
| Functional Activity | Co-immunoprecipitation or enzyme-linked immunosorbent assay (ELISA) |
Best practices derived from AT1 receptor antibody validation studies .
At1g66320 Gene Context
The At1g66320 locus encodes a protein of unknown function in Arabidopsis thaliana. Key genomic features:
| Feature | Detail |
|---|---|
| Chromosomal Location | Chromosome 1 (TAIR10 annotation) |
| Gene Length | ~1–2 kb (typical for Arabidopsis genes) |
| Putative Function | Uncharacterized (requires experimental validation) |
Based on TAIR10 genome annotation and general plant gene structures .
Research Gaps and Opportunities
-
Functional Characterization: No published studies link At1g66320 to specific biological pathways.
-
Antibody Specificity: Requires validation against recombinant At1g66320 protein or knockout mutants.
-
Cross-species Reactivity: Potential utility in other Brassicaceae species (e.g., Brassica napus) remains untested.
Inferred from limitations in AT1 receptor antibody validation and plant antibody development challenges .
Comparative Analysis of Antibody Design
The At1g66320 Antibody’s design mirrors established monoclonal antibodies, such as the anti-beta-actin antibody (66009-1-Ig) , which demonstrates:
Data synthesized from Cusabio and beta-actin antibody specifications .
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
