At3g12840 Antibody

At3g12840 Gene Overview

The gene At3g12840 encodes a F-box/FBD-like domain protein in Arabidopsis thaliana (thale cress) . Key features include:

F-box proteins typically function as substrate-recognition components of E3 ubiquitin ligase complexes, marking proteins for proteasomal degradation.

Hypothetical At3g12840 Antibody

An antibody targeting the At3g12840 protein would likely be used to:

• Detect endogenous protein levels in Arabidopsis tissues via Western blotting or immunohistochemistry.

• Study its interaction partners in ubiquitination pathways (e.g., co-immunoprecipitation).

• Validate gene-editing outcomes (e.g., CRISPR knockouts).

Potential Technical Challenges

• Specificity: F-box domains are structurally conserved; cross-reactivity with unrelated F-box proteins is possible.

• Antigen Design: Antibodies require epitopes in variable regions (e.g., N-/C-termini) to avoid cross-reactivity .

Antibody Development Pipeline

Recommendations for Future Work

To advance research on At3g12840:

1. Generate Recombinant Protein: Express and purify At3g12840 for immunization or phage display .

2. Leverage Phage/Yeast Display: Screen libraries for high-affinity binders .

3. Cross-Reactivity Screening: Test against other F-box proteins (e.g., At1g27360, At5g46260) .