At3g49020 Antibody

Research Applications

The At3g49020 Antibody is employed in studies investigating:

• Auxin Signaling Pathways: Auxin is a key phytohormone regulating plant growth and development. The At3g49020 gene is implicated in auxin-responsive pathways, as demonstrated in mutants with disrupted auxin signaling .

• Gene Expression Profiling: The antibody aids in quantifying At3g49020 protein levels in response to environmental or genetic perturbations.

• Mutant Phenotyping: It is used to analyze protein expression in mutants like hy5 (hypusine) and hy5 hyh (hypusine, hyh), which exhibit altered auxin responses and root morphology .

Auxin-Responsive Gene Expression

In a study on hy5 and hy5 hyh mutants, the At3g49020 gene was identified as part of a subset of auxin-responsive genes with dysregulated expression. Key observations include:

These data suggest that At3g49020 plays a role in auxin-mediated transcriptional regulation, particularly in roots .

Functional Implications

• Auxin Signaling Defects: In hy5 mutants, auxin-induced gene expression is blunted, indicating a potential role for At3g49020 in modulating auxin signaling pathways.

• Genetic Interactions: The hy5 hyh double mutant exhibits exacerbated auxin signaling defects compared to hy5 alone, highlighting epistatic relationships between auxin-responsive genes .