At1g24881 Antibody
Description
Introduction to At1g24881 and Its Antibody
At1g24881 is a gene in Arabidopsis thaliana (thale cress) encoding an F-box family protein, which plays a role in the ubiquitin-proteasome system (UPS) for protein degradation . The At1g24881 Antibody is a research tool designed to detect this protein, enabling studies on its localization, interactions, and regulation in plant cellular processes.
Molecular Biology Techniques
The antibody is validated for:
| Method | Purpose |
|---|---|
| Western Blotting | Detecting At1g24881 protein in cell lysates or subcellular fractions. |
| Immunoprecipitation | Isolating At1g24881 complexes for interaction studies (e.g., E3 ligase partners). |
| Immunofluorescence | Localizing At1g24881 in tissues (e.g., root tips, leaves). |
Example Workflow:
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Sample Preparation: Extract proteins from Arabidopsis tissues.
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Detection: Use the antibody (1:1000–1:5000 dilution) for WB or IF.
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Validation: Confirm specificity with knockout mutants or peptide blocking.
Unresolved Questions
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Substrate Specificity: Which proteins are targeted by the At1g24881 F-box protein?
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Regulatory Mechanisms: How is At1g24881 expression controlled under stress or developmental cues?
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Functional Redundancy: Does At1g24881 overlap with other F-box proteins in Arabidopsis?
Potential Applications in Crop Improvement
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Stress Tolerance: Engineering At1g24881 to degrade negative regulators of drought/salt responses.
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Pathogen Resistance: Targeting pathogen virulence factors via the UPS.
Product Specs
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
