At1g25211 Antibody

Introduction to At1g25211 Antibody

The At1g25211 Antibody is a monoclonal immunoglobulin G (IgG) antibody targeting the F-box/kelch-repeat protein encoded by the Arabidopsis thaliana gene At1g25211 (P0DI06). This antibody is primarily used in plant research to study the protein’s role in cellular processes, including stress responses and regulatory pathways. It is produced in mice and validated for applications such as Western blot (WB) and immunoprecipitation (IP) under the AbInsure™ program .

Mechanism of Action

The antibody binds to epitopes in the N-terminal region of P0DI06, enabling detection of the protein in Arabidopsis tissues. Its specificity is driven by complementarity-determining regions (CDRs), particularly CDR3 of the heavy chain, which provides critical diversity for antigen recognition . The use of synthetic peptides as immunogens ensures precise targeting, minimizing cross-reactivity .

Western Blot (WB) Validation

The antibody demonstrates high sensitivity in WB, detecting as little as 0.01–1 ng of immunogen peptide in dot blot assays. AbInsure™ protocols recommend a starting dilution of 1:1000 for WB, ensuring reproducibility across experimental conditions .

Cold Response Studies

Bioinformatic analyses (e.g., Table S8a in ) highlight At1g25211 as a differentially expressed (DE) gene during cold stress in Arabidopsis. This suggests a potential role for P0DI06 in cold adaptation, though functional validation remains pending.

Differential Expression in Stress Responses

As shown in Table S8a , At1g25211 is among 7,302 DE genes identified in cold-exposed Arabidopsis. Key observations include:

Source:

Functional Hypotheses

The F-box/kelch-repeat domain of P0DI06 suggests involvement in proteasomal degradation pathways, possibly regulating stress-responsive transcription factors or enzymes. Further studies using the At1g25211 Antibody could clarify its role in protein turnover during stress.

Production and Validation

• Species Limitation: Mouse-derived antibodies may face challenges in cross-species applications, though the AbInsure™ program ensures validation in Arabidopsis .

• Reproducibility: Unlike recombinant antibodies (e.g., those with engineered backbones ), traditional monoclonal antibodies may exhibit lot-to-lot variability.

Cost and Accessibility

The antibody’s price ($899 + $100 shipping) and limited availability (30-day shipment ) may restrict its use in large-scale studies.

Future Directions

1. Recombinant Production: Transitioning to recombinant antibody formats could enhance scalability and consistency .

2. Functional Studies: Knockout or CRISPR-based experiments using the At1g25211 Antibody could elucidate P0DI06’s role in cold stress.

3. Cross-Platform Validation: Testing the antibody in immunofluorescence or ELISA may expand its utility.