At1g30090 Antibody

Overview of At1g30090 Antibody

The At1g30090 antibody targets the protein encoded by the Arabidopsis thaliana gene AT1G30090, a member of the galactose oxidase/kelch repeat superfamily. This antibody is utilized in plant molecular biology research to study the localization, expression, and functional roles of the AT1G30090 protein, which is implicated in oxidative stress responses and developmental processes. While no direct commercial or clinical applications are documented in the provided sources, its utility lies in basic research to elucidate plant immune and metabolic pathways .

Gene and Protein Structure

The AT1G30090 gene encodes a 519-amino-acid protein with two conserved domains:

• Galactose oxidase domain: Involved in redox reactions.

• Kelch repeat motifs: Facilitate protein-protein interactions.

Table 1: Key Features of AT1G30090

Data sourced from ThaleMine .

Functional Studies

While no studies directly using the At1g30090 antibody are cited in the provided materials, analogous research on plant antibodies (e.g., AMP1 antibody targeting rTAPA in astrocytes ) suggests potential applications:

• Immunolocalization: Mapping protein expression in Arabidopsis tissues.

• Western blotting: Detecting AT1G30090 protein levels under stress conditions.

• Knockout validation: Confirming gene silencing in mutant plants.

Hypothetical Roles in Plant Physiology

Based on domain homology:

• Oxidative stress response: Galactose oxidase domains are linked to redox regulation.

• Pathogen defense: Kelch repeats mediate interactions with immune signaling proteins .

Comparative Analysis with Related Antibodies

Antibodies targeting plant proteins often face challenges such as cross-reactivity or epitope masking. For example:

• The AMP1 antibody recognizes both α-actinin and TAPA in denatured states but only TAPA in native conditions .

• Monoclonal antibodies like APG777 are engineered for extended half-life and optimized effector functions .

These precedents highlight the importance of rigorous validation for At1g30090 antibody specificity.

Limitations and Future Directions

No peer-reviewed studies or commercial data directly referencing the At1g30090 antibody were identified in the provided sources. Future research should:

1. Validate antibody specificity using knockout lines.

2. Explore AT1G30090’s role in plant-pathogen interactions, leveraging techniques like transcriptome profiling .

3. Investigate structural determinants of antibody-antigen binding to minimize off-target effects .