At1g51550 Antibody

Overview of At1g51550 Antibody

The At1g51550 Antibody is a mouse-derived monoclonal antibody targeting the N-terminal region of the F-box/kelch-repeat protein encoded by the At1g51550 gene in Arabidopsis thaliana. This gene is associated with regulatory roles in protein degradation pathways, likely involving ubiquitination. The antibody is designed for immunological applications, including Western blotting (WB) and immunoprecipitation (IP), under the AbInsure™ program, which guarantees performance in specific experimental conditions .

Research Applications and Utility

The At1g51550 Antibody is primarily used to study:

• Protein Stability: F-box/kelch-repeat proteins often regulate substrate ubiquitination in SCF (SKP1-CUL1-F-box) E3 ligase complexes. This antibody could track At1g51550’s role in targeting specific substrates for degradation .

• Protein-Protein Interactions: IP applications may identify binding partners, such as kelch-repeat domain interactions or SCF complex components .

• Localization Studies: WB analysis could determine subcellular distribution (e.g., cytoplasmic vs. nuclear) under stress conditions.

Limitations and Considerations

• Specificity: While the antibody is validated for WB, cross-reactivity with homologous proteins in other species (e.g., Arabidopsis lyrata) is not explicitly confirmed.

• Detection Sensitivity: Requires high target protein concentrations (0.01–1 ng), limiting utility in low-abundance samples .

• Experimental Design: Optimal performance depends on pre-treatment with protease inhibitors (e.g., MG132) to preserve ubiquitinated intermediates, as shown in analogous studies .

Potential Research Directions

1. Ubiquitination Studies:

   • Use the antibody to immunoprecipitate At1g51550 and identify polyubiquitinated substrates, similar to methods applied to CHS (chalcone synthase) in parsley .

2. Functional Analysis in Stress Responses:

   • Investigate At1g51550’s role in stress-induced protein turnover, leveraging Arabidopsis mutants or overexpression lines.

3. Structural Insights:

   • Combine with anti-ubiquitin antibodies to map At1g51550’s interaction with E2/E3 ligases or substrate proteins .