At1g64840 Antibody

Research Applications and Findings

The At1g64840 protein is implicated in Arabidopsis immune responses, particularly in defense against powdery mildew pathogens. Key studies include:

• Role in Plant Immunity: At1g64840 interacts with defense regulators such as ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), PHYTOALEXIN DEFICIENT 4 (PAD4), and SENESCENCE-ASSOCIATED GENE 101 (SAG101), which are critical for salicylic acid (SA)-mediated resistance pathways .

• Genetic Interactions: Mutational analyses revealed that At1g64840 contributes to basal immunity, with knockout plants showing compromised resistance to fungal pathogens .

• Protein Localization: Western blot studies using this antibody have helped characterize the expression patterns of At1g64840 under stress conditions, though specific subcellular localization data remain limited .

Antibody Validation and Specificity Considerations

While the At1g64840 antibody has been used in Arabidopsis research, broader lessons from antibody validation studies highlight critical considerations:

• Specificity Challenges: Independent validation is essential, as commercially available antibodies for related targets (e.g., angiotensin receptors) have demonstrated cross-reactivity or nonspecific binding in mammalian systems .

• Recommended Controls: Include knockout plant lines (e.g., AT1G64840 mutants) in Western blot and immunohistochemistry experiments to confirm target specificity.

• Functional Assays: Pair antibody-based detection with phenotypic analyses (e.g., pathogen susceptibility assays) to verify biological relevance .

Future Research Directions

• Structural Characterization: Resolve the tertiary structure of At1g64840 to define its interaction domains.

• Pathogen Interaction Studies: Investigate how At1g64840 modulates effector-triggered immunity in Arabidopsis.

• Cross-Species Reactivity: Test homologs in crop plants (e.g., Brassica species) for conserved immune functions.