At1g80440 Antibody
Description
Overview of At1g80440 Antibody
The antibody targets the AT1G80440 gene product, identified as KMD1 (KISS ME DEADLY 1), a component of the SCF-type E3 ubiquitin ligase complex. KMD1 interacts with type-B ARABIDOPSIS RESPONSE REGULATOR (ARR) proteins, which are negative regulators of cytokinin signaling .
Research Applications
The antibody is primarily used in molecular studies to investigate:
-
Cytokinin Signaling: KMD1 mediates the degradation of type-B ARR proteins, which suppress cytokinin-responsive genes. Loss of KMD1 activity leads to cytokinin hypersensitivity .
-
Phenylpropanoid Pathway: KMD1 regulates phenylalanine ammonia lyase (PAL) stability, influencing flavonoid and lignin biosynthesis .
-
Protein-Protein Interactions: Co-immunoprecipitation assays confirm interactions between KMD1 and ARR proteins .
Cytokinin Signaling Regulation
Phenylpropanoid Metabolism
Evolutionary Context
| Study Focus | Methodology | Key Results |
|---|---|---|
| KMD family evolution | BLAST and phylogenetic analysis | KMD1 belongs to a conserved F-box protein family in plants, with orthologs in Brassica and Medicago . |
Western Blotting
Co-Immunoprecipitation
-
Procedure: HA-tagged KMD1 pulled down with GFP-tagged ARR proteins in protoplasts .
-
Outcome: Confirmed physical interaction between KMD1 and ARR1/ARR12 .
