At2g21680 Antibody

Introduction to At2g21680 Antibody

The At2g21680 antibody is a polyclonal reagent specifically developed for detecting the Arabidopsis thaliana (mouse-ear cress) protein encoded by the At2g21680 gene. This antibody is generated through recombinant protein immunization and is validated for use in enzyme-linked immunosorbent assay (ELISA) and western blot (WB) applications .

Immunogen and Cross-Reactivity

The immunogen is a recombinant protein corresponding to the full-length At2g21680 sequence (UniProt ID: Q9SIJ3). Specificity testing confirms reactivity with Arabidopsis thaliana samples, with no reported cross-reactivity to non-target species under standard experimental conditions .

ELISA

• Usage: Optimal dilutions must be determined experimentally.

• Purpose: Quantification of At2g21680 protein in lysates or purified samples .

Western Blot

• Usage: Requires optimization for concentration and blocking conditions.

• Purpose: Detection of At2g21680 protein in Arabidopsis thaliana extracts, with an expected band size corresponding to the target’s molecular weight .

Product Development and Availability

• Production: Custom-made upon order, with a lead time of 14–16 weeks.

• Quality Control: Validated for specificity via affinity purification and reactivity testing .

Research Implications

While direct studies utilizing the At2g21680 antibody are not detailed in publicly available literature, its design supports investigations into:

• Gene Function: Elucidating the role of At2g21680 in Arabidopsis thaliana cellular processes.

• Protein Localization: Subcellular tracking via WB or immunofluorescence (pending further validation).