At2g22050 Antibody

Experimental Applications

The antibody's primary uses in plant research include:

• Protein detection: Western blot analysis of Arabidopsis protein extracts

• Interaction studies: Identification of F-box/kelch protein complexes

• Subcellular localization: Immunofluorescence microscopy in plant tissues

Recent methodological advances in antibody validation (as demonstrated in general antibody research ) suggest optimal protocols should include:

1. Pre-absorption controls with recombinant At2g22050

2. Parallel testing with Arabidopsis knockout mutants

3. Multiplexed detection using secondary antibodies with distinct fluorophores

Biological Context of Target Protein

The At2g22050 gene encodes a protein containing:

• F-box domain: Mediates protein-protein interactions in ubiquitination pathways

• Kelch repeats: Implicated in substrate recognition and scaffolding functions

Comparative analysis of kelch-repeat proteins reveals conserved structural features:

Research Limitations and Future Directions

While commercial availability is established , peer-reviewed studies specifically using this antibody remain limited. Current applications extrapolate from:

• General F-box/kelch protein functionality

• Arabidopsis antibody validation frameworks

Critical research gaps include:

• Crystal structure data for antigen-antibody complexes

• In planta functional studies using immunoprecipitation-mass spectrometry

• Cross-species reactivity profiles beyond Arabidopsis thaliana