At2g44130 Antibody

Biological Function of At2g44130 (KFB39)

The At2g44130 gene encodes a kelch domain-containing F-box protein (KFB39) that belongs to the KISS ME DEADLY (KMD) family. KFB39 functions as a component of the SCF (Skp1-Cullin-F-box) ubiquitin ligase complex, which targets specific proteins for degradation via the 26S proteasome .

• Interaction with PAL Isozymes: KFB39 physically interacts with PAL3 and PAL4, key enzymes in the phenylpropanoid pathway. This interaction destabilizes PAL proteins, thereby modulating lignin, flavonoid, and anthocyanin biosynthesis .

• Phylogenetic Grouping: KFB39 shares 62.8% amino acid identity with AtKFB50 and forms a clade with AtKFB01, AtKFB20, and AtKFB50, indicating functional redundancy in PAL regulation .

Applications of the At2g44130 Antibody

The antibody is used in molecular biology techniques to study KFB39’s localization, interactions, and functional roles:

Research Insights

• Regulation of PAL Stability: Overexpression of KFB39 reduces PAL protein levels, while RNA interference (RNAi)-mediated downregulation of KFB39 in atkfb01/20/50 triple mutants increases PAL accumulation by ~30%, enhancing phenylpropanoid synthesis .

• Phenotypic Effects: Silencing KFB39 in atkfb01/20/50 mutants elevates anthocyanin levels 4-fold and lignin content by ~15%, with visible purple pigmentation in leaves and stems .

Mechanistic Implications

KFB39’s role in PAL degradation highlights its importance in balancing phenylpropanoid pathway flux. By tagging PAL for proteasomal degradation, KFB39 modulates:

• Lignin Synthesis: Affects cell wall structure in mature plants .

• Flavonoid/Anthocyanin Production: Influences stress responses and UV protection .

References

1. Cusabio. Custom Antibodies. [Available at: https://www.cusabio.com/catalog-62-1498.html]

2. PLOS ONE. Genome-wide survey of F-box/Kelch proteins and TaAFR localization. [DOI: 10.1371/journal.pone.0250479]

3. ThaleMine. Gene: AT2G44130. [Available at: https://bar.utoronto.ca/thalemine/gene:At2g44130]

4. PMC. Down-regulation of Kelch Domain-Containing F-Box Protein in Arabidopsis. [DOI: 10.1093/plphys/plv139]

5. Plant Physiology. Additive Effects of AtKFB39 on PAL Stability. [DOI: 10.1104/pp.114.251561]