At3g10510 Antibody

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Description

Antibody Overview

The At3g10510 antibody is a primary antibody used to detect and study the protein product of the At3g10510 gene in biochemical assays. Key attributes include:

AttributeDetails
TargetGalactose oxidase/kelch repeat superfamily protein (Uniprot ID: Q9CAE9) .
SpeciesArabidopsis thaliana (Mouse-ear cress) .
Product FormLiquid (2ml/0.1ml volume) .
ApplicationsWestern blot, immunoprecipitation, and protein interaction studies .

This antibody is part of a broader catalog of plant-specific primary antibodies, including those targeting proteins like APS1 (AT5G61400) and AT4G36750 .

Key Features

  • Epitope Recognition: The antibody binds to epitopes on the At3g10510 protein, enabling detection in assays like Western blot or immunoprecipitation .

  • Isotype: Not explicitly stated, but primary antibodies for plant proteins are typically polyclonal due to high diversity requirements .

Research Applications

The At3g10510 antibody is utilized in studies investigating:

ApplicationDetails
Protein LocalizationIdentifies subcellular localization (e.g., cytoplasm, nucleus) via immunofluorescence .
Gene Expression AnalysisQuantifies protein levels in response to environmental stressors or developmental cues .
Protein InteractionsMaps interactions with other kelch repeat-containing proteins via co-IP .

For example, studies on Arabidopsis histone demethylases have used antibodies at dilutions of 1:170 to analyze post-translational modifications .

Characterization and Validation

The antibody’s performance is validated through:

MethodOutcome
Western BlotDetects target protein in lysates, with expected molecular weight (MW) .
ELISAMeasures binding affinity and specificity against recombinant At3g10510 .
ImmunoprecipitationConfirms pull-down of At3g10510 and interacting partners .

Commercial suppliers like Cusabio (e.g., CSB-PA887256XA01DOA) provide antibodies with pre-validated protocols .

Research Findings and Implications

  • Gene Function: At3g10510 is linked to processes like epigenetic regulation (e.g., histone demethylation) .

  • Comparative Biology: Kelch repeat proteins in plants may share functional analogies with human proteins involved in ubiquitination pathways .

Limitations and Future Directions

  • Specificity Challenges: Cross-reactivity with homologous kelch repeat proteins requires validation .

  • Therapeutic Potential: While not directly therapeutic, insights into plant kelch proteins could inform drug discovery in human diseases .

Product Specs

Buffer
Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Form
Liquid
Lead Time
14-16 week lead time (made-to-order)
Synonyms
At3g10510 antibody; F13M14.21 antibody; F18K10.8Putative F-box/kelch-repeat protein At3g10510 antibody
Target Names
At3g10510
Uniprot No.

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