The At3g10510 antibody is a primary antibody used to detect and study the protein product of the At3g10510 gene in biochemical assays. Key attributes include:
Attribute
Details
Target
Galactose oxidase/kelch repeat superfamily protein (Uniprot ID: Q9CAE9).
Species
Arabidopsis thaliana (Mouse-ear cress).
Product Form
Liquid (2ml/0.1ml volume).
Applications
Western blot, immunoprecipitation, and protein interaction studies.
This antibody is part of a broader catalog of plant-specific primary antibodies, including those targeting proteins like APS1 (AT5G61400) and AT4G36750.
Key Features
Epitope Recognition: The antibody binds to epitopes on the At3g10510 protein, enabling detection in assays like Western blot or immunoprecipitation.
Isotype: Not explicitly stated, but primary antibodies for plant proteins are typically polyclonal due to high diversity requirements.
Research Applications
The At3g10510 antibody is utilized in studies investigating:
Application
Details
Protein Localization
Identifies subcellular localization (e.g., cytoplasm, nucleus) via immunofluorescence.
Gene Expression Analysis
Quantifies protein levels in response to environmental stressors or developmental cues.
Protein Interactions
Maps interactions with other kelch repeat-containing proteins via co-IP.
For example, studies on Arabidopsis histone demethylases have used antibodies at dilutions of 1:170 to analyze post-translational modifications.
Characterization and Validation
The antibody’s performance is validated through:
Method
Outcome
Western Blot
Detects target protein in lysates, with expected molecular weight (MW).
ELISA
Measures binding affinity and specificity against recombinant At3g10510.
Immunoprecipitation
Confirms pull-down of At3g10510 and interacting partners.
Commercial suppliers like Cusabio (e.g., CSB-PA887256XA01DOA) provide antibodies with pre-validated protocols.
Research Findings and Implications
Gene Function: At3g10510 is linked to processes like epigenetic regulation (e.g., histone demethylation).
Comparative Biology: Kelch repeat proteins in plants may share functional analogies with human proteins involved in ubiquitination pathways.
Limitations and Future Directions
Specificity Challenges: Cross-reactivity with homologous kelch repeat proteins requires validation.
Therapeutic Potential: While not directly therapeutic, insights into plant kelch proteins could inform drug discovery in human diseases.