At3g17540 Antibody
Description
Introduction to At3g17540 Antibody
The At3g17540 Antibody is a polyclonal antibody targeting the protein encoded by the At3g17540 gene in Arabidopsis thaliana (Mouse-ear cress). This gene is annotated as an F-box/kelch-repeat protein, a class of proteins involved in substrate recognition for ubiquitination and subsequent proteasomal degradation . The antibody (Product Code: CSB-PA862896XA01DOA) is widely used in plant molecular biology research to study protein localization, expression dynamics, and functional roles under various stress conditions.
Role in Arsenic Stress Response
A 2021 study published in The Plant Cell utilized the At3g17540 Antibody to investigate arsenic (As) stress mechanisms in Arabidopsis :
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Gene Regulation: At3g17540 was identified as part of the transcriptional network activated under arsenite (AsIII) exposure, suggesting a role in heavy metal detoxification.
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Protein Localization: Immunoblotting confirmed the antibody’s specificity for detecting At3g17540 in root tissues under As stress.
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Functional Insight: As an F-box protein, At3g17540 likely mediates ubiquitination of stress-response regulators, influencing phosphate transporter (PHT1;1) activity during arsenic exposure .
Interaction Networks
While direct interactors remain under study, F-box proteins like At3g17540 typically form SCF complexes (Skp1-Cullin-F-box) to tag substrates for degradation. This mechanism is critical for:
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Phosphate homeostasis
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Stress signaling pathways
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Developmental regulation
Applications in Plant Biology
The At3g17540 Antibody has been instrumental in:
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Stress Adaptation Studies: Uncovering molecular responses to environmental toxins like arsenic .
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Protein Turnover Analysis: Investigating ubiquitination-dependent regulation of nutrient transporters.
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Transgenic Line Validation: Confirming gene-edited or overexpression lines in Arabidopsis mutants.
Future Research Directions
Key unanswered questions include:
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Identification of At3g17540’s substrate(s) in arsenic signaling.
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Cross-species conservation of its stress-response role.
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Structural characterization of its kelch-repeat domains.
This antibody remains a critical tool for advancing plant stress biology and protein degradation studies.
