At3g22870 Antibody

Expression Analysis

The At3g22870 Antibody enables detection of its target protein in various experimental conditions. For example:

These data suggest the protein is downregulated under drought stress (dS/) and upregulated in rpt2a mutants (nS/), indicating a role in stress adaptation or proteasomal regulation .

Stress Response

The observed downregulation in WT plants under drought stress (dS/) aligns with hypotheses that F-box/kelch-repeat proteins modulate stress-responsive transcription factors. For instance, such proteins may regulate the degradation of transcriptional activators/repressors during abiotic stress.

Proteasomal Regulation

In rpt2a mutants (defective in the 26S proteasome), the upregulation of AT3G22870 suggests a compensatory mechanism to restore protein homeostasis. This mirrors findings in other proteasome-deficient models, where F-box proteins are often upregulated to counteract protein aggregation .

Limitations and Future Directions

• Limited Functional Data: No direct studies on the antibody’s role in disrupting protein degradation pathways.

• Species-Specific Utility: Primarily validated in Arabidopsis; cross-reactivity with other plant species is unconfirmed.

• Future Research:

  • CRISPR Knockouts: Combine antibody-based detection with gene-edited mutants to elucidate functional roles.

  • Omics Integration: Pair with transcriptomic/proteomic data to identify downstream targets.