At3g27910 Antibody

Definition and Basic Characteristics

The At3g27910 Antibody is a custom-generated antibody targeting the Arabidopsis thaliana protein encoded by the gene locus At3g27910 (UniProt ID: Q9LK86). This immunoglobulin is designed for specific detection and analysis of the At3g27910 protein in plant biology research. It belongs to a class of antibodies used to study protein interactions, subcellular localization, and functional roles in cellular processes .

Role in Protein-Protein Interactions

The At3g27910 Antibody has been utilized to study interactions involving the HAP3/5 subunit of the HAP complex, a transcription factor critical for plant development. Key findings include:

• Coimmunoprecipitation Studies:

  • At3g27910 (HAP5a) interacts with CONSTANS (CO), a flowering-time regulator, in in vitro assays. This interaction was confirmed using GAD:CO fusion proteins and immunoprecipitation with anti-GAD antibodies .

  • The interaction does not involve At HAP2a (a subunit of the HAP complex), suggesting a distinct functional role for HAP5a in CO-mediated pathways .

• Mutation Analysis:

  • Mutations in the CO CCT domain (e.g., co-9) do not disrupt binding to At HAP3a or At HAP5a, indicating that these interactions are independent of flowering-time regulation .

Applications in Plant Biology

• Protein Localization: The antibody aids in tracking the subcellular distribution of At3g27910, particularly in nuclear or cytoplasmic compartments.

• Functional Studies: It facilitates investigations into the HAP complex’s role in phytochrome signaling and photosynthetic gene regulation, which are critical for light-responsive development in plants .

Comparison with Related Antibodies

The At3g27910 Antibody is part of a broader suite of Arabidopsis-specific antibodies, including:

Data derived from commercial catalogs and functional annotations .

Future Research Directions

• Structural Studies: Cryo-EM or X-ray crystallography could elucidate the binding interface between At3g27910 and CO, providing insights into transcriptional regulation.

• Therapeutic Potential: While not directly therapeutic, insights from At3g27910 studies may inform strategies to engineer plant growth traits for agricultural applications.

References and Methodological Considerations

• Immunoprecipitation Protocols: Use of GAD-specific antibodies (e.g., anti-Gal4) for pull-down assays ensures specificity in detecting interactions .

• Cross-Reactivity: Testing against homologous plant proteins (e.g., At HAP5b) is recommended to confirm target specificity .