At3g43710 Antibody
Description
Production and Validation
The antibody is produced using antigen-affinity purification, ensuring high specificity. Validation data includes:
| Parameter | Detail |
|---|---|
| Host Species | Rabbit |
| Purification Method | Antigen Affinity Purified |
| Tested Applications | ELISA, Western Blot (WB) |
| Storage Temperature | -20°C or -80°C (avoid repeated freeze) |
| Lead Time | 14–16 weeks (made-to-order) |
Research Applications
This antibody is integral to:
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Protein Expression Analysis: Quantifying At3g43710 levels under experimental conditions .
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Subcellular Localization: Mapping protein distribution in plant tissues.
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Mechanistic Studies: Investigating interactions with other biomolecules, leveraging structural insights from analogous antibodies .
Recent studies on methylated lysine-specific antibodies highlight the importance of epitope specificity, a principle directly relevant to At3g43710’s design .
Technical Considerations
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Specificity: Validated for Arabidopsis thaliana; users must confirm cross-reactivity for other species.
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Storage Stability: Maintains activity for years at recommended temperatures .
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Experimental Optimization: Titration required for WB/ELISA to minimize nonspecific binding .
Future Directions
Advances in antibody engineering, such as phage display and AI-driven design , may enhance future iterations of At3g43710 antibodies. Current limitations include long production times (14+ weeks) and the need for empirical validation .
Product Specs
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
