At4g33290 Antibody
Description
Definition and Background
The At4g33290 Antibody is a polyclonal rabbit-derived immunoglobulin (IgG) designed to specifically target the F-box/kelch-repeat protein encoded by the At4g33290 gene in Arabidopsis thaliana (thale cress), a model plant species used in molecular biology studies . This antibody serves as a critical tool for detecting and analyzing the protein’s expression, localization, and functional interactions in plant research.
Antibody Structure
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Isotype: IgG, characterized by a Y-shaped structure with two antigen-binding fragments (Fab) and a crystallizable fragment (Fc) .
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Target Epitopes: Binds to the recombinant At4g33290 protein, likely recognizing conserved regions within the F-box and kelch-repeat domains .
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Purification: Antigen-affinity purified to ensure specificity and minimize cross-reactivity .
Key Features
Generation Process
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Immunization: Rabbits immunized with recombinant At4g33290 protein to induce antibody production.
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Purification: Affinity chromatography using the immunogen to isolate specific IgG molecules .
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Testing: Validated for use in ELISA (enzyme-linked immunosorbent assay) and Western Blot (WB) to detect the target protein .
Performance Metrics
While detailed binding affinity (Kd) or specificity data are not publicly disclosed, the antibody is optimized for:
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Sensitivity: Detects At4g33290 in crude lysates or purified preparations.
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Cross-Reactivity: Limited to Arabidopsis thaliana based on host specificity .
Primary Uses
| Application | Purpose |
|---|---|
| ELISA | Quantitative analysis of At4g33290 protein levels in plant extracts . |
| Western Blotting | Confirming protein identity and size (~85 kDa, estimated from SDS-PAGE purity) . |
Research Context
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Gene Function Studies: Investigating the role of F-box/kelch-repeat proteins in plant growth, stress responses, or protein degradation pathways .
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Protein-Protein Interactions: Identifying binding partners via co-IP (immunoprecipitation) or pull-down assays (indirectly inferred from antibody utility) .
Comparative Analysis: Polyclonal vs. Monoclonal Antibodies
Note: Polyclonal antibodies often provide broader detection in complex samples, while monoclonal antibodies offer higher precision .
Key Insights
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Validation: Successfully used in plant protein detection workflows without reported cross-reactivity with non-Arabidopsis species .
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Technical Challenges: Limited data on antibody performance in immunohistochemistry (IHC) or live-cell imaging, suggesting a focus on biochemical assays .
Gaps in Literature
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Binding Affinity: No published Kd values or competition assays available.
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Functional Studies: No direct evidence linking antibody use to downstream functional analyses (e.g., knockdown experiments).
