At2g29910 Antibody

Introduction to At2g29910 Antibody

The At2g29910 Antibody is a polyclonal antibody designed to detect the protein encoded by the At2g29910 gene in Arabidopsis thaliana (mouse-ear cress). This gene belongs to the F-box/LRR-repeat protein family, which is implicated in ubiquitination processes and protein degradation pathways . The antibody is primarily used in plant molecular biology research to study protein localization, expression levels, and functional interactions.

Key Properties

Recombinant Protein Information

The target protein (At2g29910) is produced in multiple expression systems:

• Hosts: E. coli, yeast, baculovirus, or mammalian cells .

• Function: The F-box/LRR-repeat protein belongs to the F-box/RNI-like superfamily, which regulates protein turnover via the ubiquitin-proteasome system .

Detection Methods

The antibody is validated for:

• Western Blot (WB): Identifies At2g29910 protein in lysates or tissue extracts .

• ELISA: Quantifies protein levels in Arabidopsis samples .

Specificity and Validation

Commercial antibodies require rigorous validation due to risks of nonspecific binding. For example:

• Cross-Reactivity: Ensure no reactivity with unrelated proteins (e.g., confirm via knockout controls) .

• Host-Specificity: Primarily validated for Arabidopsis thaliana; cross-reactivity with other species is unconfirmed .

Technical Recommendations

• Optimal Conditions: Follow manufacturer protocols for buffer composition, blocking agents, and incubation times.

• Controls: Use Arabidopsis wild-type and knockout samples to confirm target specificity .

References

1. Cusabio Antibody Catalog

2. MyBioSource Product Page

3. Nonspecificity of Commercial Antibodies