At3g48880 Antibody

Biological Role of the AT3G48880 Protein

The AT3G48880 gene encodes SNIPER4, an F-box protein that facilitates the degradation of nucleotide-binding leucine-rich repeat (NLR) immune receptors via the SCF^CRP1 complex . Key functional insights include:

SNIPER4 acts as a substrate receptor in the SCF E3 ubiquitin ligase complex, tagging NLR receptors for proteasomal degradation to prevent hyperactive immune signaling .

Validation Considerations

Antibody specificity is critical given the homology among F-box proteins. Best practices from monoclonal antibody research recommend:

• Knockout (KO) validation: Use Arabidopsis mutant lines to confirm target absence in Western blots .

• Functional assays: Pair with ubiquitination assays to verify SCF^CRP1 activity .

• Cross-reactivity checks: Test against related F-box proteins (e.g., SNIPER1–3).

Research Implications

The At3g48880 antibody could advance studies on:

• Plant-pathogen interactions: Elucidate NLR receptor regulation during immune activation.

• Ubiquitination dynamics: Map degradation pathways in plant stress responses.

• CRISPR-edited lines: Validate gene-editing outcomes in Arabidopsis.

Future Directions

• Structural characterization: Use cryo-EM or X-ray crystallography to resolve SNIPER4-MUSE13/14 interactions .

• Multispecies cross-reactivity: Test antibody utility in crops like Brassica or Oryza.