At3g58920 Antibody
Description
Definition and Target
The At3g58920 antibody is a rabbit-derived polyclonal antibody raised against the recombinant Arabidopsis thaliana At3g58920 protein (UniProt ID: Q9LXR0). It is primarily used to detect and study the expression, localization, and function of the At3g58920 protein in plant research .
Research Applications
The antibody is validated for:
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Western Blot: Identification of the ~30 kDa At3g58920 protein in plant lysates .
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Specificity: Confirmed reactivity with Arabidopsis thaliana samples; no cross-reactivity data for other species are reported .
Limitations and Considerations
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Research-Grade Use: Explicitly labeled "For Research Use Only," excluding diagnostic or therapeutic applications .
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Validation Gaps: Peer-reviewed publications specifically using this antibody are not cited in available sources, highlighting the need for independent validation in experimental settings.
Comparative Analysis with Related Antibodies
The table below contrasts At3g58920 with other Arabidopsis-targeted antibodies from the same vendor :
| Antibody Target | Product Code | UniProt ID | Applications |
|---|---|---|---|
| At3g58920 | CSB-PA888856XA01DOA | Q9LXR0 | ELISA, WB |
| At4g13960 | CSB-PA521272XA01DOA | O23257 | ELISA, IHC |
| FBL19 | CSB-PA888882XA01DOA | Q9M096 | WB, IF |
Future Directions
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Functional Studies: Employing this antibody to elucidate At3g58920’s role in plant development or stress responses.
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Omics Integration: Pairing with transcriptomic or proteomic datasets to contextualize its expression.
Product Specs
Components: 50% Glycerol, 0.01M PBS, pH 7.4
Q&A
Basic Research Questions
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What experimental applications are validated for At3g58920 antibody in Arabidopsis studies?
This polyclonal antibody is specifically validated for ELISA and western blot (WB) applications in Arabidopsis thaliana research . For WB:-
Use 10-20 µg of total protein lysate per lane
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Employ Tris-glycine or Bis-Tris gels (4-12% gradient recommended)
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Optimize blocking with 5% non-fat milk or BSA in TBST
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How should At3g58920 antibody be stored for maximum stability?
The antibody requires storage at -20°C or -80°C in 50% glycerol/PBS buffer with 0.03% Proclin 300 . Best practices:-
Aliquot into single-use volumes (10-20 µL)
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Avoid >3 freeze-thaw cycles
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Centrifuge tubes briefly before opening after thawing
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What controls are essential when using this antibody for knockout validation?
Advanced Research Questions
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How to resolve nonspecific bands in western blots using At3g58920 antibody?
Troubleshooting protocol:-
Reduce antibody concentration (test 1:500 to 1:2000 dilutions)
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Increase stringency:
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Add 0.1% Tween-20
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Use high-salt wash buffer (150-300 mM NaCl)
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Pre-absorb with E. coli lysate expressing unrelated proteins
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What experimental designs address contradictory results between ELISA and western blot data?
Implement orthogonal verification through:-
Immunoprecipitation-MS: Confirm target co-precipitation
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Genetic complementation: Express tagged At3g58920 in knockout lines
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Cellular fractionation: Verify subcellular localization consistency
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Technical Specifications Table
| Parameter | Detail |
|---|---|
| Host Species | Rabbit |
| Immunogen | Recombinant At3g58920 (Full length) |
| Cross-reactivity | Arabidopsis thaliana specific |
| Purification | Antigen-affinity chromatography |
| Long-term Storage | -80°C in 50% glycerol |
