At4g13960 Antibody
Description
Research Applications
The At4g13960 Antibody is optimized for immunological assays to study the At4g13960 protein in Arabidopsis thaliana. While specific experimental data are not publicly available, its intended uses include:
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ELISA: Detection of At4g13960 in solution-phase assays.
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Western Blotting (WB): Identification of the protein in lysates or purified samples.
Considerations for Use
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Storage and Handling:
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Store at -20°C or -80°C to maintain stability.
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Avoid repeated freeze-thaw cycles to prevent degradation.
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Use within the recommended shelf life for optimal performance.
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Species-Specific Reactivity:
The antibody is validated for Arabidopsis thaliana and may not cross-react with homologous proteins in other species. -
Non-Therapeutic Use:
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For Research Only: Not approved for diagnostic or therapeutic applications.
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Comparative Context
The At4g13960 Antibody is one of several Arabidopsis-specific antibodies listed by Cusabio, including:
| Antibody | Uniprot No. | Species | Code |
|---|---|---|---|
| At4g13960 | O23257 | Arabidopsis thaliana | CSB-PA521272XA01DOA |
| At3g59160 | Q9LX55 | Arabidopsis thaliana | CSB-PA888849XA01DOA |
| At3g58920 | Q9LXR0 | Arabidopsis thaliana | CSB-PA888856XA01DOA |
| ... | ... | ... | ... |
Research Gaps and Future Directions
While the At4g13960 Antibody is a specialized tool for Arabidopsis studies, current literature lacks detailed experimental validation (e.g., epitope mapping, binding kinetics, or cross-reactivity data). Further studies are needed to:
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Characterize its specificity in diverse Arabidopsis genotypes.
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Explore applications in plant stress response or developmental biology.
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Compare performance with other anti-At4g13960 antibodies.
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Q&A
The At4g13960 antibody is a polyclonal reagent developed for studying Arabidopsis thaliana proteins in research settings. Below are structured FAQs addressing both fundamental and advanced methodological considerations, derived from technical specifications and research applications .
Advanced Methodological Challenges
Resolving inconsistent western blot signals across developmental stages
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Problem: Variable band intensities in floral vs. vegetative tissues
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Solution workflow:
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Verify tissue-specific protein degradation (protease inhibitor cocktail)
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Test alternative extraction buffers (e.g., RIPA vs. Tris-urea)
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Optimize transfer conditions for low-MW variants (15% SDS-PAGE, 90 min transfer)
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5. Addressing cross-reactivity in non-target species
While species reactivity is specific to Arabidopsis thaliana , homology screening reveals:
| Species | Sequence Identity | Recommended Dilution |
|---|---|---|
| Brassica napus | 82% | 1:5000 |
| Thellungiella halophila | 79% | Not recommended |
Quantitative ELISA normalization strategies
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Spike-in recombinant protein (0.1-10 ng/μl range)
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Correct for plate-to-plate variance using:
Technical Troubleshooting Guide
| Issue | Probable Cause | Verification Protocol |
|---|---|---|
| High background in WB | Insufficient blocking | Test 3% BSA vs. 5% milk |
| Multiple bands | Protein isoforms | Perform PNGase F treatment |
| No signal | Epitope masking | Antigen retrieval (95°C, 5 min citrate buffer) |
Experimental Design Considerations
For time-course studies:
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Sampling Interval: 0, 6, 12, 24, 48 h post-treatment
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Replicates: n=5 biological, 3 technical
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Controls:
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Knockout line (CRISPR-Cas9 generated)
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Constitutive expressor (35S::At4g13960)
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