At5g54820 Antibody
Description
Introduction to At5g54820 Antibody
The At5g54820 Antibody is a polyclonal rabbit antibody designed to detect the protein encoded by the At5g54820 gene in Arabidopsis thaliana (mouse-ear cress). This gene belongs to the F-box/LRR-repeat protein family, which is associated with ubiquitin-mediated protein degradation pathways. The antibody serves as a critical tool in plant molecular biology research, enabling the detection and characterization of the At5g54820 protein in experimental settings.
Gene and Protein Overview
At5g54820 encodes a putative F-box/LRR-repeat protein (UniProt ID: Q9FFU7), which likely participates in protein-protein interactions and ubiquitination processes. F-box proteins are components of E3 ubiquitin ligase complexes, targeting specific substrates for degradation. While the exact function of At5g54820 remains under investigation, its structural similarity to other F-box proteins suggests roles in cellular regulation, stress responses, or developmental processes in Arabidopsis.
Antibody Characteristics
The At5g54820 Antibody is characterized by:
| Parameter | Details |
|---|---|
| Type | Polyclonal (rabbit) |
| Reactivity | Arabidopsis thaliana (mouse-ear cress) |
| Purification | Antigen-affinity chromatography |
| Applications | ELISA, Western blot (WB), and immunoprecipitation (IP) |
Applications in Research
The antibody is validated for:
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ELISA: Detecting recombinant or endogenous At5g54820 protein in Arabidopsis extracts.
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Western Blot: Identifying the protein’s molecular weight (~45 kDa, based on similar F-box proteins).
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Immunoprecipitation: Enriching At5g54820 for downstream analysis (e.g., mass spectrometry or interaction studies).
Cross-Reactivity and Specificity
While the antibody is marketed for Arabidopsis thaliana, cross-reactivity with homologs in other plant species has not been explicitly tested. Researchers should validate specificity for their experimental system, particularly in heterologous expression studies.
Research Findings and Challenges
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Western Blot Validation: Protocols similar to those described for V5-tagged proteins (e.g., SDS-PAGE, PVDF membrane transfer, and HRP-conjugated secondary antibodies) may be applicable.
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Immunoprecipitation: Strategies analogous to DEFA5 antibody workflows (e.g., Protein A/G bead pull-downs) could be adapted.
Product Specs
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
