At1g11620 Antibody

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Description

Protein Target and Antibody Characteristics

The antibody targets the protein At1g11620 (UniProt ID: Q9SAB5), a 363-amino acid protein in Arabidopsis thaliana. Key features include:

  • Gene Name: At1g11620

  • Protein Length: 363 amino acids

  • Sequence: Includes regions with F-box, leucine-rich repeat (LRR), and kelch-repeat domains, which suggest roles in protein degradation and signal transduction .

The antibody is available in multiple formats, including monoclonal and polyclonal variants:

Antibody TypeDescriptionSource
Monoclonal (Mouse)Combinations of mAbs targeting N-terminal, C-terminal, or mid-region sequences
Polyclonal (Rabbit)Antigen-affinity purified, raised against recombinant At1g11620 protein

Antibody Variants and Applications

The At1g11620 Antibody is optimized for specific experimental techniques:

Monoclonal Antibody Combinations

Three synthetic peptide-based mouse monoclonal antibody (mAb) combinations are available:

  1. X-Q9SAB5 -N: Targets N-terminal sequence (residues 1–120).

  2. X-Q9SAB5 -C: Targets C-terminal sequence (residues 244–363).

  3. X-Q9SAB5 -M: Targets mid-region sequence (residues 121–243).

ParameterN-Terminus (X-Q9SAB5 -N)C-Terminus (X-Q9SAB5 -C)Mid-Region (X-Q9SAB5 -M)
ELISA Titer10,00010,00010,000
WB Detection~1 ng target protein~1 ng target protein~1 ng target protein
Availability30 days30 days30 days

These combinations enable high-specificity detection in Western blotting (WB) and enzyme-linked immunosorbent assay (ELISA) .

Polyclonal Antibody (Rabbit)

  • Immunogen: Recombinant Arabidopsis thaliana At1g11620 protein .

  • Reactivity: Specific to Arabidopsis thaliana; no cross-reactivity reported .

  • Form: Liquid, non-conjugated, stored at -20°C or -80°C .

Purchasing Options

Commercial availability includes pre-packaged combinations and customizable solutions:

PackageComponentsPriceDelivery
X2 -Q9SAB5N- and C-terminal mAb mixes$89930 days
Single CombinationChoose N, C, or M mAb mix$59930 days

For large-scale projects, custom antibody development is offered, with options to tailor epitope specificity or cross-reactivity .

Technical Considerations

  • Epitope Determination: Deconvolution services are available ($100 per combination) to identify individual mAb epitopes .

  • Storage: Avoid repeated freeze-thaw cycles; store at -20°C/-80°C .

  • Species Limitations: Validated only for Arabidopsis thaliana; cross-reactivity with other species is unconfirmed .

Product Specs

Buffer
Preservative: 0.03% Proclin 300
Composition: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
At1g11620 antibody; F25C20.23Putative F-box/LRR-repeat/kelch-repeat protein At1g11620 antibody
Target Names
At1g11620
Uniprot No.

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