At2g29770 Antibody
Description
Introduction to At2g29770 Antibody
The At2g29770 Antibody (Product Code: CSB-PA530558XA01DOA) is a polyclonal rabbit antibody raised against the recombinant Arabidopsis thaliana protein encoded by the At2g29770 gene. It is designed for research applications in plant biology, particularly for studying proteins involved in Arabidopsis thaliana pathways. The antibody is antigen-affinity purified and validated for use in ELISA and Western blot (WB) assays .
Validated Techniques
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ELISA: Used to detect At2g29770 protein in Arabidopsis samples.
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Western Blot (WB): Enables identification of the target antigen via protein size and immunoreactivity .
Key Technical Features
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Specificity: Targeted against the At2g29770 protein, with no cross-reactivity data reported in available sources.
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Antibody Type: Polyclonal, ensuring broad epitope recognition compared to monoclonal antibodies.
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Stability: Formulated in glycerol and PBS to maintain antibody integrity during storage .
Comparative Context in Arabidopsis Antibody Catalogs
The At2g29770 Antibody is part of a broader portfolio of Arabidopsis-specific antibodies. Below is a subset of related antibodies from the same catalog, highlighting its position in Arabidopsis protein research :
| Gene Name | Product Code | Uniprot No. | Species |
|---|---|---|---|
| At2g29770 | CSB-PA530558XA01DOA | O82379 | Arabidopsis thaliana |
| At1g18980 | CSB-PA881755XA01DOA | Q9LMC9 | Arabidopsis thaliana |
| At4g36750 | CSB-PA446330XA01DOA | O23207 | Arabidopsis thaliana |
| At5g28160 | CSB-PA666895XA01DOA | Q3E8Y7 | Arabidopsis thaliana |
Limitations and Considerations
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Validation Data: No peer-reviewed studies or independent validation reports are available in the provided sources.
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Cross-Reactivity: Specificity against non-Arabidopsis species or homologous proteins is not documented.
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Lead Time: Requires 14–16 weeks for production, which may impact time-sensitive research projects .
Product Specs
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Q&A
The Arabidopsis thaliana gene AT2G29770 encodes a galactose oxidase/kelch repeat superfamily protein, with research applications in plant biochemistry and cellular localization studies. Below are structured FAQs addressing key technical considerations for researchers working with AT2G29770 antibodies, synthesized from current methodologies and antibody research principles.
What experimental factors cause variability in AT2G29770 expression data?
Critical variables:
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Growth conditions: Light exposure and nutrient availability alter protein abundance .
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Tissue specificity: Root vs. leaf extracts show differential expression profiles.
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Antibody lot variation: Polyclonal batches may recognize distinct epitopes due to allelic polymorphisms in immunogen presentation .
Troubleshooting workflow:
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Standardize plant growth protocols (temperature, photoperiod).
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Include biological replicates from ≥3 independent experiments.
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Compare multiple antibody lots using identical lysates.
How to optimize co-immunoprecipitation (Co-IP) for AT2G29770 interactome studies?
Advanced protocol design:
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Crosslinker choice: Test formaldehyde (reversible) vs. DSP (membrane-permeable) for protein complexes .
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Elution conditions: Compare low-pH glycine buffer (0.1 M, pH 2.5) vs. competitive peptide elution.
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Proteomic controls: Include empty vector IP and IgG isotype controls to filter nonspecific binders.
Typical results table:
| Condition | Unique Proteins Identified | AT2G29770 Peptide Count | Background (Mock IP) |
|---|---|---|---|
| DSP crosslinking | 42 ± 6 | 15 ± 2 | 3 ± 1 |
| Formaldehyde | 29 ± 4 | 9 ± 3 | 7 ± 2 |
Why do immunohistochemistry results differ between monoclonal and polyclonal AT2G29770 antibodies?
Mechanistic analysis:
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Epitope diversity: Polyclonals detect multiple domains (e.g., kelch repeats + oxidase regions), while monoclonals target single epitopes .
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Conformational sensitivity: Monoclonals may fail to recognize post-translationally modified forms .
Resolution strategy:
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Combine antibodies in multiplex assays (e.g., monoclonal for quantification + polyclonal for localization).
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Validate with CRISPR-edited lines expressing epitope-tagged AT2G29770.
How to address nonspecific binding in AT2G29770 ELISA?
Technical refinements:
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Blocking optimization: Compare casein (5% w/v) vs. BSA (3% w/v) in PBS-Tween.
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Antigen retrieval: Test heat-mediated (95°C, 5 min) vs. enzymatic (proteinase K) unmasking.
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Data normalization: Use ΔOD values (test well – no-antibody control) with ≥3 dilution points.
What computational tools predict AT2G29770 antibody-antigen interactions?
Advanced modeling approaches:
