FBLN2 Antibody
Description
Introduction to FBLN2 Antibodies
FBLN2 antibodies are immunological reagents designed to detect and quantify the Fibulin-2 protein in research and diagnostic contexts. These antibodies are used to investigate FBLN2's roles in ECM organization, cell adhesion, and cancer biology .
Role in Mammary Gland Development
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Expression Dynamics: FBLN2 is highly expressed in terminal end buds (TEBs) during murine mammary gland puberty, localizing to cap cells and stromal regions .
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Hormonal Regulation: Estrogen (E2) and progesterone (P) upregulate Fbln2 mRNA by 3.6–4.0 fold in pubertal mice .
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Matrix Interactions: FBLN2 enhances epithelial cell adhesion to laminin, vitronectin, and fibronectin but does not directly affect migration .
Clinical Significance in Cancer
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Breast Cancer Prognosis: High Fbln2 mRNA levels correlate with improved distant metastasis-free survival in lymph node-negative (HR = 0.71, P = 0.03) and intermediate-grade (HR = 0.76, P = 0.05) breast cancers .
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Tumor Suppression: FBLN2 knockdown in mammary epithelial cells disrupts BM integrity and reduces integrin β1 expression, promoting tumor invasiveness .
Table 1: Staining Patterns in Mammary Gland TEBs vs. Ducts
| Structure | % Age Score | Intensity Score | Quickscore |
|---|---|---|---|
| TEB | 6 | 3 | 18 |
| Duct | 4 | 1 | 4 |
Table 2: Clinical Relevance of Fbln2 mRNA in Breast Cancer
| Patient Subgroup | Hazard Ratio (HR) | P-value |
|---|---|---|
| LN-negative | 0.71 | 0.03 |
| Grade II | 0.76 | 0.05 |
| Grade III | 1.32 | 0.023 |
Technical Considerations
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Validation: Orthogonal RNAseq and immunofluorescence are used to confirm specificity .
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Storage: Most antibodies are stable at −20°C in glycerol-based buffers .
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Limitations: Cross-reactivity with other fibulin family members requires careful validation .
Future Directions
Product Specs
Target Background
- In vitro studies using cultured breast cancer cell lines have demonstrated that Fibulin-2 is a more favorable substrate for ADAMTS-5 compared to ADAMTS-4. Degradation of Fibulin-2 is associated with enhanced invasive potential in T47D, MCF-7, and SK-BR-3 cells. PMID: 28099917
- Following retinal detachment, levels of fibulin 2 significantly increase in the retinal pigment epithelium, suggesting a direct involvement of fibulin 2 in the re-attachment of the retina to the retinal pigment epithelium. PMID: 24692557
- A study identified and validated a novel target of miR-1, Fibullin-2 (Fbln2), a secreted protein implicated in extracellular matrix remodeling. PMID: 23612897
- Two variants in the fibulin2 gene have been associated with lower systolic blood pressure and a reduced risk of hypertension. PMID: 22912785
- Research provides evidence that the FBLN2 short isoform (FBLN2S) plays a significant tumor-suppressive and anti-angiogenic role in nasopharyngeal carcinoma (NPC). PMID: 21743496
