At5g10820 Antibody

What is At5g10820 Antibody?

The At5g10820 antibody is a polyclonal reagent developed for detecting the Arabidopsis thaliana (Mouse-ear cress) protein encoded by the At5g10820 gene. This antibody targets a protein associated with folate transport and metabolic pathways, though its precise biological role remains under investigation .

Functional Characterization

• Expression Studies: At5g10820 was tested alongside other Arabidopsis folate-biotin transporter (FBT) family proteins (At5g25040, At5g25050, At1g79710, At5g54860) in Escherichia coli and Leishmania donovani mutants. Despite homology to folate transporters, At5g10820 showed no detectable folate or pterin transport activity in these systems .

• Structural Insights: Mutagenesis studies on conserved residues in FBT proteins revealed critical regions for substrate binding. While At5g10820 shares structural motifs with functional transporters (e.g., central cavity α-helices H1, H4, H7, H10), it lacks conserved residues essential for folate binding .

Comparative Analysis

Target Identification

• At5g10820 antibodies enable subcellular localization studies to determine tissue-specific expression patterns in Arabidopsis.

• Used in Western blotting and immunofluorescence to validate gene-editing outcomes in plant biotechnology .

Functional Genomics

• Critical for exploring evolutionary divergence in FBT proteins, as At5g10820’s lack of transport activity contrasts with functional homologs like Leishmania major BT1 .

Limitations and Future Directions

• Functional Unclearance: The absence of transport activity in heterologous systems suggests At5g10820 may require plant-specific cofactors or function in non-canonical pathways .

• Antibody Utility: While essential for detecting protein expression, this antibody has not yet been used in structural studies (e.g., cryo-EM or X-ray crystallography) .