FBX10 Antibody
Description
FBX10 Antibody Overview
FBX10 antibodies target the protein encoded by the FBXO10 gene, which is part of the F-box protein family. Key characteristics include:
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Function: Facilitates protein ubiquitination and degradation via SCF (SKP1–CUL1–F-box protein) complexes, impacting cellular processes like apoptosis and metabolism .
Orthologs exist in mice, rats, zebrafish, and chimpanzees, enabling cross-species studies .
3.1. Ubiquitin-Proteasome System Studies
FBX10 antibodies are critical for examining SCF complex-mediated protein degradation. For example:
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Mechanistic Insights: FBXO10 recruits substrates for ubiquitination, influencing cell cycle regulation and stress responses .
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Disease Links: Dysregulation of FBXO10 is implicated in cancers and neurodegenerative disorders due to aberrant protein turnover .
3.2. Therapeutic Development
Recent advancements in antibody engineering, such as noncovalent catenation (e.g., fusion with SDF-1α), enhance binding avidity by up to 234-fold. This innovation improves detection sensitivity in assays targeting cell surface antigens like CD20 and HER2 .
3.3. Technical Validation
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Specificity: Affinity-purified antibodies show minimal cross-reactivity in human and rat tissues (e.g., positive detection in rat brain lysates) .
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Buffer Compatibility: Most formulations include glycerol and preservatives (e.g., sodium azide) for long-term stability .
Future Directions
Emerging techniques like computational antibody design (e.g., ROSETTA modeling) promise to refine FBX10 antibody efficacy for high-precision applications in diagnostics and therapeutics . Additionally, exploring FBXO10's interaction networks could unveil novel targets for diseases linked to protein homeostasis.
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Q&A
Here’s a structured FAQ for researchers working with FBXO10 antibodies, emphasizing experimental rigor and methodological depth:
Advanced Research Questions
How to resolve discrepancies between Western blot and immunohistochemistry (IHC) results?
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Troubleshooting workflow:
What strategies improve FBXO10 antibody performance in low-abundance samples?
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Signal amplification:
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Pre-analytical variables:
How to design FBXO10 functional studies in protein ubiquitination assays?
| Parameter | Recommendation | Rationale |
|---|---|---|
| Ubiquitin linkage | Use K48- or K63-specific antibodies | FBXO10’s role in apoptotic pathways |
| Proteasome inhibition | Treat with MG-132 (10 μM, 6 hr) | Stabilizes ubiquitinated substrates |
| Co-IP partners | Screen for SCF complex components (e.g., SKP1, CUL1) | Validates E3 ligase activity |
Data Contradiction Analysis
Interpreting conflicting results in FBXO10 localization studies
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Case example:
