FBX6 Antibody
Description
Introduction to FBXO6 Antibody
FBXO6 (F-box protein 6) is a substrate recognition component of the SCF (Skp1-Cullin1-F-box) ubiquitin ligase complex, targeting glycoproteins for degradation via its F-box-associated (FBA) domain . The FBXO6 antibody is a research tool used to detect and study this protein’s role in cellular processes, including DNA damage response, replication stress, and cancer progression . While the gene symbol is FBXO6, the antibody is sometimes referred to as "FBX6" in literature, though this appears to be a typographical error.
Applications of FBXO6 Antibody
The antibody is validated for:
Western Blot (WB)
Immunohistochemistry (IHC)
| Parameter | Details |
|---|---|
| Dilution | 1:50–1:500 (human cancer tissues) |
| Antigen Retrieval | TE buffer (pH 9.0) or citrate buffer (pH 6.0) |
| Reactivity | Human and mouse samples |
Ovarian Cancer Progression
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Overexpression in Tumors: Elevated FBXO6 levels correlate with poor survival in advanced-stage ovarian cancer .
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Mechanism: FBXO6 degrades RNASET2, promoting cell proliferation, migration, and invasion. Silencing RNASET2 partially reverses these effects .
Chemotherapy Resistance
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Chk1 Regulation: FBXO6-mediated Chk1 degradation terminates S-phase checkpoints, enhancing sensitivity to replication stress agents like camptothecin .
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Biomarker Potential: FBXO6 expression predicts tumor responsiveness to anticancer drugs .
Endoplasmic Reticulum Stress
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Q&A
Here’s a structured collection of FAQs for researchers working with FBX6 antibody in academic contexts, adhering to your specifications for scientific depth, methodological rigor, and avoidance of commercial topics. The questions are categorized into basic and advanced tiers, with data-driven insights and actionable recommendations.
Advanced Research Questions
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How to resolve contradictory FBX6 localization data between IF and subcellular fractionation?
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Troubleshooting steps:
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Confirm antibody cross-reactivity via mass spectrometry of immunoprecipitated proteins.
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Validate fractionation purity using compartment-specific markers (e.g., Lamin B1 for nuclear, GAPDH for cytoplasmic).
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Test alternative fixation methods (e.g., methanol for cytoplasmic epitopes).
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What statistical approaches are suitable for FBX6 expression quantification in heterogeneous tissues?
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Method: Use spatial transcriptomics coupled with immunohistochemistry (IHC) to correlate mRNA and protein levels.
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Analysis: Apply mixed-effects models to account for tissue heterogeneity, reporting adjusted p-values and confidence intervals.
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How to design a multiplex assay combining FBX6 detection with other ubiquitin ligase components?
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Workflow:
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Optimize sequential staining: FBX6 (mouse monoclonal, Alexa Fluor 488) → FBXW7 (rabbit polyclonal, Alexa Fluor 594).
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Include isotype controls for both channels.
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Validate colocalization via Pearson’s correlation coefficient (threshold: ≥0.6).
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Data Contradiction Analysis Framework
Scenario: FBX6 shows elevated expression in RNA-seq but not in Western blot.
| Possible Cause | Investigative Action | Outcome Example |
|---|---|---|
| Post-translational regulation | Perform cycloheximide chase assay | Half-life <2 hours |
| Alternative splicing | Design primers spanning exon junctions | Detect truncated isoforms |
