FCGR1A Antibody
Description
FCGR1A Antibody: Definition and Biological Context
FCGR1A (FcγRIa/CD64) is a 72 kDa transmembrane glycoprotein expressed on monocytes, macrophages, dendritic cells, and activated granulocytes . It binds monomeric IgG with high affinity, mediating immune functions such as phagocytosis, antigen presentation, and cytokine production . FCGR1A antibodies enable the detection, quantification, and functional analysis of this receptor in research and clinical settings.
Types of FCGR1A Antibodies
FCGR1A antibodies vary in host species, clonality, conjugates, and applications. Key examples include:
Table 1: Select FCGR1A Antibodies and Properties
Key Features:
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Monoclonal Antibodies (e.g., Clone 10.1 ): Target extracellular epitopes, ideal for FACS and functional assays.
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Polyclonal Antibodies (e.g., ABIN6261700 ): Detect endogenous FCGR1A across species, suited for WB and immunohistochemistry.
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Conjugated Variants: FITC or APC conjugates enable real-time tracking in live-cell imaging .
Research Applications
FCGR1A antibodies are instrumental in:
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Immune Cell Profiling: Quantifying CD64 expression on monocytes/macrophages to assess activation states .
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Disease Mechanisms: Linking FCGR1A variants (e.g., rs1050204) to sarcoidosis susceptibility and lung function decline .
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Cancer Immunology: Correlating FCGR1A expression with immune infiltration levels in cervical (CESC), kidney (KIRC), and skin cancers (SKCM) .
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Therapeutic Development: Targeting CD64 for autoimmune disease interventions or vaccine adjuvants .
Table 2: FCGR1A in Disease and Immune Regulation
Technical Considerations
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Epitope Sensitivity: Some antibodies (e.g., Clone 10.1) lose binding after formalin fixation .
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Species Cross-Reactivity: Most antibodies target human FCGR1A, with limited reactivity in non-primates .
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Functional Assays: Antibodies like mAB 197 induce FcγRI-mediated signaling, useful for studying receptor activation .
Future Directions
Emerging research focuses on:
Product Specs
Product Science Overview
CD64, also known as Fc gamma receptor I (FcγRI), is a high-affinity receptor for the Fc region of immunoglobulin G (IgG). It plays a crucial role in the immune system by mediating various immune responses, including phagocytosis, antibody-dependent cellular cytotoxicity (ADCC), and the release of inflammatory mediators. CD64 is primarily expressed on monocytes, macrophages, dendritic cells, and activated granulocytes .
CD64 is a 72 kDa type I transmembrane glycoprotein. It associates with a signaling FcRγ homodimer to form the functional high-affinity FcγRI complex . This receptor is unique among Fcγ receptors due to its high affinity for monomeric IgG, particularly the IgG1 and IgG3 subclasses . This high affinity allows CD64 to bind IgG even at low concentrations, making it a critical player in immune responses.
CD64 is involved in various immune functions:
- Phagocytosis: CD64 mediates the ingestion and destruction of pathogens by phagocytic cells.
- ADCC: It facilitates the killing of antibody-coated target cells by natural killer (NK) cells and other cytotoxic cells.
- Inflammatory Response: CD64 triggers the release of pro-inflammatory cytokines and chemokines, contributing to the inflammatory response .
CD64 has emerged as an attractive target for immunotherapy, particularly in the treatment of chronic inflammatory diseases. Dysregulated macrophages expressing CD64 play a key role in the initiation and maintenance of these diseases . Targeting CD64 with specific antibodies or immunotherapeutic agents can selectively eliminate these disease-causing macrophages, offering a promising strategy for treating conditions such as rheumatoid arthritis, atopic dermatitis, and autoimmune disorders .
Mouse anti-human CD64 antibodies are widely used in research and clinical applications. These antibodies can be used in various techniques, including flow cytometry, immunohistochemistry, Western blotting, immunocytochemistry, and ELISA . They are valuable tools for studying CD64 expression, function, and its role in disease processes.
