FAD4L2 Antibody
Description
FAD4L2: Functional Context in Lipid Biosynthesis
FAD4L2 is a gene associated with fatty acid desaturation and oil biosynthesis. In studies on oilseed crops (e.g., sesame), FAD4L2 exhibits elevated expression during early seed development stages (10 and 20 days post-anthesis, DPA), alongside other lipid-related genes like WSD1/DGAT and LPAAT . This suggests its role in regulating unsaturated fatty acid composition during seed maturation.
| Gene | Expression Peak (DPA) | Function | Source |
|---|---|---|---|
| FAD4L2 | 10–20 DPA | Fatty acid desaturation | |
| WSD1/DGAT | 10–20 DPA | Diacylglycerol acyltransferase | |
| LPAAT | 10–20 DPA | Lysophosphatidic acid acyltransferase |
Antibody Development for Lipid-Related Proteins
For proteins like FAD4L2, antibody generation typically follows standardized protocols:
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Immunogen Selection: Recombinant FAD4L2 protein or synthetic peptides representing conserved regions.
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Hybridoma Technology: Fusion of immunized mouse spleen cells with myeloma cells (e.g., P3x63Ag8U1) .
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Screening: ELISA, Western blot, and immunohistochemistry to validate specificity .
Example Workflow
Challenges in Antibody Characterization
Antibody efficacy depends on rigorous validation:
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ELISA vs. Functional Assays: ELISA positives may fail in immunohistochemistry or Western blotting due to epitope conformational changes .
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Isoform Specificity: FAD4L2 antibodies must distinguish between paralogs (e.g., FAD4) to avoid off-target effects .
Future Directions
While no FAD4L2-specific antibodies are documented, emerging strategies include:
Product Specs
Components: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Target Background
FAD4L2 is a fatty acid desaturase enzyme responsible for producing specific molecular species of chloroplast phosphatidylglycerol. Its function is to catalyze the introduction of a trans double bond near the carboxyl group of palmitic acid, specifically within the phosphatidylglycerol molecule esterified at the sn-2 glyceryl carbon position.
Q&A
Based on analysis of recent peer-reviewed studies, here are key research-focused FAQs addressing FAdV-4 fiber-1 protein antibodies (3B5 and 6H9) with methodological insights:
Advanced Research Questions
How to resolve contradictory results between epitope linearity and functional activity?
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Experimental strategy:
What methods identify mitochondrial metabolic alterations in antibody-treated cancer models?
How to design humanization strategies preserving FABP4-neutralizing efficacy?
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CDR grafting approach:
Human Germline VH FR VL FR Binding Affinity (KD) IGHV1-46 S74N Y49F 2.1 nM → 3.8 nM IGHV3-23 - - 2.1 nM → 15.4 nM
Technical Challenge Resolution
Addressing tumor microenvironment heterogeneity in antibody efficacy trials
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Multi-parametric analysis:
