At1g78760 Antibody
Description
Definition and Target Identification
At1g78760 Antibody refers to an immunoglobulin reagent designed to detect and bind the protein product of the Arabidopsis thaliana gene locus AT1G78760. This gene encodes a F-box/RNI-like superfamily protein, which is implicated in ubiquitin-mediated proteolysis pathways. The antibody’s primary function is to enable the visualization, quantification, or functional disruption of this protein in experimental settings .
2.1. AT1G78760 Gene Characteristics
2.2. Antibody Development Challenges
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Epitope Specificity: No published data confirm the exact epitope targeted by this antibody.
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Cross-Reactivity: Potential overlap with structurally similar F-box proteins (e.g., MIPS1, MIPS2, MIPS3) necessitates rigorous validation .
3.1. Documented Use Cases
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Immunolocalization: Used to study subcellular localization in Arabidopsis endosperm .
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Protein Interaction Studies: Facilitates co-immunoprecipitation assays to identify binding partners.
3.2. Technical Considerations
| Parameter | Recommendation |
|---|---|
| Host Species | Likely raised in rabbit or mouse (common for plant antibodies) |
| Clonality | Polyclonal (assumed due to lack of hybridoma data) |
| Validation | Western blot, ELISA, and knockout line controls advised |
Gaps in Current Knowledge
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Structural Data: No crystal structures or epitope mapping studies available.
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Functional Studies: Limited linkage to phenotypic outcomes in Arabidopsis mutants.
Future Directions
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High-Throughput Screening: Integrate with CRISPR-edited Arabidopsis lines to validate target specificity.
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Phylogenetic Analysis: Compare homologs in crop species (e.g., Brassica) to assess conserved epitopes.
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
